CLONING AND EXPRESSION OF A DEVELOPMENTALLY REGULATED PROTEIN THAT INDUCES MITOGENIC AND NEURITE OUTGROWTH ACTIVITY
CLONING AND EXPRESSION OF A DEVELOPMENTALLY REGULATED PROTEIN THAT INDUCES MITOGENIC AND NEURITE OUTGROWTH ACTIVITY
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DOI：
10.1126/science.2270483
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发表时间：
1990-12-21
期刊：
影响因子：
56.9
通讯作者：
DEUEL, TF
中科院分区：
文献类型：
--
作者：
LI, YS;MILNER, PG;DEUEL, TF
从牛子宫中分离的肝素结合促有丝分裂蛋白与从新生大鼠脑中分离的蛋白共享NH 2-末端氨基酸序列。牛、人和大鼠基因的cDNA已被分离出来，并编码与已知生长或神经营养因子无关的非常保守的蛋白质，尽管在分化的小鼠胚胎癌细胞中发现与视黄酸诱导转录物的预测序列有近50%的同一性。瞬时表达该蛋白的COS-7细胞的裂解物对NRK细胞具有促有丝分裂作用，并从胚胎大鼠脑细胞的混合培养物中启动神经突生长。编码这种蛋白质的RNA转录本广泛分布于组织中，并受到发育调控。这种蛋白质以前被命名为肝素结合生长因子（HBGF）-8，现在更名为多效营养素（PTN），以反映其多种活性。PTN可能是发育调节细胞因子家族的第一个成员。
A heparin binding mitogenic protein isolated from bovine uterus shares NH2-terminal amino acid sequence with a protein isolated from newborn rat brain. The cDNA''s of the bovine, human, and rat genes have been isolated and encode extraordinarily conserved proteins unrelated to known growth or neurotrophic factors, although identity of nearly 50 percent has been found with the predicted sequence of a retinoic acid induced transcript in differentiating mouse embryonal carcinoma cells. Lysates of COS-7 cells transiently expressing this protein were mitogenic for NRK cells and initiated neurite outgrowth from mixed cultures of embryonic rat brain cells. RNA transcripts encoding this protein were widely distributed in tissues and were developmentally regulated. This protein, previously designated as heparin binding growth factor (HBGF)-8, is now renamed pleiotrophin (PTN) to reflect its diverse activities. PTN may be the first member of a family of developmentally regulated cytokines.