Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia
Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia
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锥虫属的分子特征和系统发育分析。
DOI：
10.1016/j.ijppaw.2019.04.009
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Sawa
中科院分区：
文献类型：
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作者：
Qiu Yongjin;Kajihara Masahiro;Harima Hayato;Hang'ombe Bernard Mudenda;Nakao Ryo;Hayashida Kyoko;Mori-Kajihara Akina;Changula Katendi;Eto Yoshiki;Ndebe Joseph;Yoshida Reiko;Takadate Yoshihiro;Mwizabi Daniel;Kawabata Hiroki;Simuunza Martin;Mweene Aaron;Sawa
蝙蝠锥虫由来自70多种蝙蝠的30多种锥虫组成。最近的研究表明，蝙蝠在非洲大陆向非洲-古北生态区和新北生态区的陆生哺乳动物传播锥虫中起着重要作用。然而，蝙蝠锥虫的多样性，分布和进化仍然不清楚。为了更好地了解它们的进化，需要更多来自不同地点的蝙蝠锥虫的遗传数据。在一次调查中。的蝙蝠栖息在赞比亚的洞穴，我们观察到鞭毛虫寄生虫从5的43血培养。糖体甘油醛3-磷酸脱氢酶基因（gGAPDH; 572 bp）和18 S核糖体RNA基因（18 S rRNA基因; 1，079 - 1，091 bp）的序列分析和系统发育分析表明，所有的锥虫属都属于锥虫属的Cruziclade。其中3个和2个与T. ConorhiniandT. dionisii，分别。本研究首次提供了锥虫的遗传学资料。赞比亚蝙蝠的数量。
Bat trypanosomes consist of more than 30 trypanosome species from over 70 species of bats. Recent studies suggest that bats play a role in disseminating trypanosomes from African continent to the terrestrial mammals both in the Afrotropic-Palearctic Ecozones and Nearctic Ecozone. However, the diversity, distribution, and evolution of bat trypanosomes are still unclear. To better understand their evolution, more genetic data of bat trypanosomes from a variety of locations are required. During a survey ofBorreliaspp. of bats inhabiting a cave in Zambia, we observed flagellate parasites from 5 of 43 hemocultures. Sequence and phylogenetic analyses of the glycosomal glyceraldehyde 3-phosphate dehydrogenase gene (gGAPDH; 572 bp) and the 18S ribosomal RNA gene (18S rRNA gene; 1,079–1,091 bp) revealed that all wereTrypanosoma spp.belonged to theTrypanosoma cruziclade. Three and two of them exhibited the similarity withT. conorhiniandT. dionisii, respectively. The present study provides the first genetic data onTrypanosomaspp. of bats inhabiting Zambia.