Reduced lesions in chickens with Clostridium perfringens-induced necrotic enteritis by Lactobacillus fermentum 1.2029
Reduced lesions in chickens with Clostridium perfringens-induced necrotic enteritis by Lactobacillus fermentum 1.2029
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发酵乳杆菌 1.2029 减少产气荚膜梭菌引起的坏死性肠炎鸡的病变
DOI：
10.3382/ps.2012-02548
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发表时间：
2012-12-01
期刊：
影响因子：
4.4
通讯作者：
Yao, J. H.
中科院分区：
文献类型：
--
作者：
Cao, L.;Yang, X. J.;Yao, J. H.
以往的研究表明乳酸杆菌具有抗炎作用，但乳酸杆菌的保护作用及其抑制鸡肠道坏死性肠炎（NE）的机制尚未完全阐明。在本研究中，我们选择了一个益生菌菌株，发酵乳杆菌，1.2029，它具有良好的粘附能力，并在低pH值和胆盐的高存活率。本研究的目的是研究L。1.2029对鸡NE的抑制作用。选用1日龄爱拔益加肉鸡240只，随机分为3个试验组：（1）非攻毒对照组，（2）产气荚膜梭菌攻毒组，（3）C.产气荚膜梭菌攻毒+ L. fermentum，1.2029群。在实验过程中，每天向组III口服发酵乳杆菌1.2029（1.0 mL/d，10（8）cfu/mL），并相应地用相同体积的PBS接种所有未感染的对照鸡。组II给予产气荚膜梭菌（第1天0.5 mL，第14 ~ 21天1.0 mL，10（8）cfu/mL）。在28 d时，进行大体NE病变评分。从每个实验组中的24只鸡收集约2cm的回肠段，并固定在4%（wt/vol）中性缓冲福尔马林溶液中用于组织学评分。还收集回肠粘膜样品用于通过实时PCR进行mRNA分析。结果表明，L.发酵菌1.2029降低了鸡NE病变的严重程度。组织学评分显示L. fermentum，1.2029还可以减轻NE对鸡的炎症损伤。测定细胞因子和Toll样受体（TLR）的变化。发现发酵菌1.2029在NE感染的鸡中增加白细胞介素-10水平并降低干扰素-γ和TLR 2水平。结果表明，L.发酵菌1.2029能够通过改变细胞因子和TLR的表达水平来调节肠粘膜免疫反应，减轻炎症。
Previous studies have demonstrated that Lactobacillus has anti-inflammatory properties, but the protective functions of Lactobacillus and mechanisms of inhibition of necrotic enteritis (NE) in the intestines of chickens have not been fully clarified. In the present study, we selected a probiotic strain, Lactobacillus fermentum, 1.2029, which has good adhesive ability and a high survival rate in low pH and bile salts. The objective of this study was to examine the anti-inflammatory properties of L. fermentum 1.2029 against NE in chickens. Two hundred forty 1-d-old male Arbor Acres broilers were blocked into 3 experimental groups as follows: (I) nonchallenge control group, (II) Clostridium perfringens challenge group, and (III) C. perfringens challenge + L. fermentum, 1.2029 group. Lactobacillus fermentum 1.2029 (1.0 mL/d, 10(8) cfu/mL) was orally administered daily to group III during the course of the experiment, and all uninfected control chickens were inoculated accordingly with the same volume of PBS. Clostridium perfringens (0.5 mL on d 1 and 1.0 mL on d 14 to 21, 10(8) cfu/mL) was administered to chickens in group II. At 28 d, scoring of gross NE lesions was performed. Ileal segments of approximately 2 cm from 24 chickens in each experimental group were collected and fixed in 4% (wt/vol) neutral-buffered formalin solution for histological scoring. Ileal mucosa samples were also collected for mRNA analysis by real-time PCR. The results showed that L. fermentum 1.2029 reduced the severity of NE lesions in chickens. Histological scores revealed that L. fermentum, 1.2029 also reduced the inflammation damage of NE in chickens. Changes in cytokines and Toll-like receptors (TLR) were determined, and L. fermentum 1.2029 was found to increase interleukin-10 levels and reduce interferon-gamma and TLR2 levels in NE-infected chickens. The results showed that L. fermentum 1.2029 was able to regulate the intestinal mucosal immure response and ameliorate inflammation by changing expression levels of cytokines and TLR.