Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon

Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon
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凯西亚松四核苷酸微卫星标记的开发

DOI:
10.1007/s12686-012-9814-3
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发表时间:
2012
影响因子:
1.1
通讯作者:
S. Shiraishi
S. Shiraishi
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
D. V. Thao;M. Yamashita;A. Watanabe;S. Shiraishi

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思茅松(Pinus kesiya Royle ex Gordon,P. kesiya)是东南亚重要的经济和生态树种之一。12个多态性四核苷酸微卫星标记,从这个物种中分离出使用双抑制聚合酶链反应(PCR)技术,这些标记的特点是在24个人收集的自然种群在越南南部。每个位点的等位基因数在2 ~ 28个之间,12个位点的期望杂合度在0.198 ~ 0.954之间,平均为0.685。这些多态性标记将成为研究野生稻保护遗传学的有用工具。kesiyaand probably可能in its closely密切related相关species种.
Pinus kesiyaRoyle ex Gordon (P. kesiya) is one of the economically and ecologically important pine species in South-East Asian. 12 polymorphic tetranucleotide microsatellite markers were isolated from this species using a dual-suppression polymerase chain reaction (PCR) technique and these markers were characterized in 24 individuals collected from a natural population in the Southern Vietnam. The number of alleles per locus ranged from 2 to 28, and expected heterozygosities of the 12 loci ranged from 0.198 to 0.954, with an average of 0.685. These polymorphic markers will serve as useful tools for studies of conservation genetics inP. kesiyaand probably in its closely related species.
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