Mdm31 and Mdm32 are inner membrane proteins required for maintenance of mitochondrial shape and stability of mitochondrial DNA nucleoids in yeast.
Mdm31 and Mdm32 are inner membrane proteins required for maintenance of mitochondrial shape and stability of mitochondrial DNA nucleoids in yeast.
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DOI：
10.1083/jcb.200410030
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发表时间：
2005-01-03
影响因子：
7.8
通讯作者：
Westermann, Benedikt
中科院分区：
文献类型：
--
作者：
Dimmer, Kai Stefan;Jakobs, Stefan;Vogel, Frank;Altmann, Katrin;Westermann, Benedikt
MDM31和MDM32基因是酿酒酵母中线粒体正常分布和形态所必需的。它们编码位于线粒体内膜中不同蛋白复合物中的两种相关蛋白。缺乏Mdm 31和Mdm 32的细胞具有巨大的球形线粒体，其内部结构高度异常。线粒体DNA（mtDNA）是不稳定的突变体，线粒体DNA的类核是无序的，它们与外膜中含有Mmm 1的复合物的关联被废除。突变的线粒体在很大程度上是不动的，导致线粒体遗传缺陷。MDM31和MDM32基因中任一个的缺失是合成致死的，MMM 1、MMM 2、MDM10和MDM12基因中任一个的缺失编码参与线粒体形态发生和mtDNA遗传的外膜蛋白。我们推测Mdm 31和Mdm 32与Mmm 1、Mmm 2、Mdm 10和Mdm 12在线粒体形态和线粒体DNA的维持中起协同作用。
The MDM31 and MDM32 genes are required for normal distribution and morphology of mitochondria in the yeast Saccharomyces cerevisiae. They encode two related proteins located in distinct protein complexes in the mitochondrial inner membrane. Cells lacking Mdm31 and Mdm32 harbor giant spherical mitochondria with highly aberrant internal structure. Mitochondrial DNA (mtDNA) is instable in the mutants, mtDNA nucleoids are disorganized, and their association with Mmm1-containing complexes in the outer membrane is abolished. Mutant mitochondria are largely immotile, resulting in a mitochondrial inheritance defect. Deletion of either one of the MDM31 and MDM32 genes is synthetically lethal with deletion of either one of the MMM1, MMM2, MDM10, and MDM12 genes, which encode outer membrane proteins involved in mitochondrial morphogenesis and mtDNA inheritance. We propose that Mdm31 and Mdm32 cooperate with Mmm1, Mmm2, Mdm10, and Mdm12 in maintenance of mitochondrial morphology and mtDNA.