REVERSE-TRANSCRIPTASE IN A CLINICAL STRAIN OF ESCHERICHIA-COLI - PRODUCTION OF BRANCHED RNA-LINKED MSDNA
REVERSE-TRANSCRIPTASE IN A CLINICAL STRAIN OF ESCHERICHIA-COLI - PRODUCTION OF BRANCHED RNA-LINKED MSDNA
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DOI：
10.1126/science.2466332
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发表时间：
1989-02-24
期刊：
影响因子：
56.9
通讯作者：
INOUYE, M
中科院分区：
文献类型：
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作者：
LAMPSON, BC;SUN, J;INOUYE, M
最初在粘细菌中检测到的分支RNA连接的多拷贝单链DNA（msDNA）现在已在大肠杆菌的临床分离株中发现。虽然在一级结构上缺乏同源性，但E.大肠杆菌msDNA在二级结构上与粘细菌msDNA相似，包括RNA和DNA之间的2“，5”-磷酸二酯键。将一个负责msDNA产生的染色体DNA片段克隆到E. coliK 12菌株，其DNS序列显示一个586个氨基酸残基的开放阅读框架（ORF）。ORF序列与逆转录病毒逆转录酶和核糖核酸酶H相似。ORF的破坏阻断了msDNA的产生，表明该基因对msDNA合成是必需的。
Branched RNA-linked multicopy single-stranded DNA (msDNA) originally detected in myxobacteria has now been found in a clinical isolate of Escherichia coli. Although lacking homology in the primary structure, the E. coli msDNA is similar in secondary structure to the myxobacterial msDNA''s, including the 2'',5''-phosphodiester linkage between RNA and DNA. A chromosomal DNA fragment responsible for the production of msDNA was cloned in an E. coli K12 strain; its DNS sequence revealed an open reading frame (ORF) of 586 amino acid residues. The ORF shows sequence similarly with retroviral reverse transcriptases and ribonuclease H. Disruption of the ORF blocked msDNA production, indicating that this gene is essential for msDNA synthesis.