The ability of blue crab (Callinectes sapidus, Rathbun 1886) to sustain aerobic metabolism during hypoxia
The ability of blue crab (Callinectes sapidus, Rathbun 1886) to sustain aerobic metabolism during hypoxia
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DOI：
10.1016/j.jembe.2015.06.003
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发表时间：
2015-10-01
影响因子：
2
通讯作者：
Small, Hamish J.
中科院分区：
文献类型：
--
作者：
Brill, Richard W.;Bushnell, Peter G.;Small, Hamish J.
为了评估成年青蟹（Callinectes sapidus）在近海栖息地日益常见的缺氧条件下的功能，临界氧水平（即，可维持有氧代谢的最低氧水平）在一定代谢率范围内使用自动呼吸流量测定法测定。通过在三种温度（17，23和28摄氏度）下进行实验，测试最近喂养的螃蟹，以及感染寄生甲藻血甲藻的螃蟹，诱导不同的代谢率。还测量了低氧对个体在强制性力竭活动后的代谢率和恢复时间以及摄食后的代谢率的影响，以确定可能影响摄食、消化和整体能量学的低氧水平。与先前发表的结果相反，发现青蟹不是部分氧构象体（即，其中代谢率福尔斯与环境氧的减少一致地下降），而是作为氧调节剂（即，具有维持恒定有氧代谢速率直到达到临界氧水平的能力）。通过这种方法，在常规代谢率下，蓝蟹与其他十足类甲壳动物一样耐缺氧，其临界氧含量中位数为-20%空气饱和度（在17和23摄氏度下）。在28摄氏度时，感染H的个体的临界氧水平随着代谢率的增加而增加。perezii，和那些最近喂养。在最高的代谢率（在28摄氏度下在最近进食的个体中测量），中值临界氧水平是-45%的空气饱和度。与后一种观察结果一致，进食或运动后的代谢率直到低于50%的空气饱和度才受到影响，尽管在该缺氧水平下最大代谢率较低。所提出的结果是一致的氧气水平影响青蟹的行为（类似于2至4毫克升（-1）），在现场和实验室设置。出版社：Elsevier B. V.
To assess the ability of adult blue crab (Callinectes sapidus) to function under the hypoxic conditions becoming increasingly common in their inshore habitats, critical oxygen levels (i.e., the minimum oxygen levels at which aerobic metabolism can be maintained) were determined over a range of metabolic rates using automated intermittent-flow respirometry. Different metabolic rates were induced by conducting experiments at three temperatures (17, 23, and 28 degrees C), testing recently fed crabs, and those infected with the parasitic dinoflagellate Hematodinium perezi. The effects of hypoxia on the metabolic rates and recovery times of individuals following enforced exhaustive activity, and metabolic rates following feeding, were also measured to determine the levels of hypoxia likely to impact feeding, digestion, and overall energetics. Contrary to previously published results, blue crab were found not to be partial oxygen conformers (i.e., where metabolic rate falls in concert with reductions in ambient oxygen), but rather to be oxygen regulators (i.e., to have the ability to maintain a constant aerobic metabolic rate until the critical oxygen level was reached). By this measure, at routine metabolic rates blue crab are as hypoxia-tolerant as other decapod crustaceans with a median critical oxygen level of -20% air saturation (at 17 and 23 degrees C). Critical oxygen levels increased in concert with the increases in metabolic rate occurring at 28 degrees C, in individuals infected with H. perezii, and those recently fed. At the highest metabolic rates (measured in recently fed individuals at 28 degrees C) median critical oxygen level was -45% air saturation. Consistent with this latter observation, metabolic rates after feeding or exercise were not compromised until below 50% air saturation, although maximum metabolic rates were lower at this level of hypoxia. The results presented are consistent with the oxygen levels shown to influence blue crab behaviors (similar to 2 to 4 mg l(-1)) in both field and laboratory settings. Published by Elsevier B.V.