Fluorescence from a single Symbiodinium cell
Fluorescence from a single Symbiodinium cell
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DOI：
10.1088/2050-6120/aaba89
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发表时间：
2018-04
影响因子：
3.2
通讯作者：
Christine Guzman;Xue Han;E. Shoguchi;Síle Nic Chormaic
中科院分区：
文献类型：
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作者：
Christine Guzman;Xue Han;E. Shoguchi;Síle Nic Chormaic
珊瑚和其共生藻类共生体之间的伙伴关系对全球环境至关重要。然而，共生菌的光合作用机制中的调控过程仍然不清楚。在这里，我们研究了聚焦的红蓝激光对单个共生体细胞的光应力的影响。测量荧光信号以显示细胞反应。增加入射激光功率或曝光时间会导致荧光强度的增加和下降。荧光强度的变化趋势与光利用效率、非光化学猝灭、光抑制和细胞修复机制有关。我们的研究为研究共生线虫细胞的光生物学和生理学提供了新的途径。
The partnership between coral and its algal symbionts, Symbiodinium, is crucial to the global environment. Yet, the regulatory process within the photosynthetic machinery of Symbiodinium is still not clearly understood. Here, we studied the influence of light stress from focussed red and blue lasers on single Symbiodinium cells. Fluorescence signals were measured to show cell response. Increasing the incident laser power or the exposure time resulted in an increase followed by a decline in fluorescence intensity. The trend of fluorescence intensity changes was associated with mechanisms of light use efficiency, non-photochemical quenching, photoinhibition, and repair of the cell. Our study provides new approaches to studying the photobiology and physiology of Symbiodinium cells.