Live Imaging of Bone Cell and Organ Cultures
Live Imaging of Bone Cell and Organ Cultures
复制标题
DOI：
10.1007/978-1-61779-415-5_26
复制
发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Veno, Patricia A.
中科院分区：
文献类型：
--
作者：
Dallas, Sarah L.;Veno, Patricia A.
在过去的二十年里，使用光和共聚焦显微镜对活细胞成像的能力取得了前所未有的进步。随着绿色荧光蛋白及其衍生物的发现和大量荧光成像探针和偶联物的发展，现在几乎可以对任何细胞内或细胞外的蛋白质或结构进行成像。传统的固定骨细胞和组织的静态成像是在特定时间点对事件的快照视图，但往往会错过正在研究的事件的动态方面。本章概述了活细胞成像方法在骨细胞和骨器官培养研究中的应用。我们没有强调成像设备的技术方面，而是把重点放在我们认为对研究者在他们自己的实验室中建立这些技术最实用的重要原则上，以及我们实验室用于骨细胞和器官培养的实时成像的详细协议。
Over the past two decades there have been unprecedented advances in the capabilities for live cell imaging using light and confocal microscopy. Together with the discovery of green fluorescent protein and its derivatives and the development of a vast array of fluorescent imaging probes and conjugates, it is now possible to image virtually any intracellular or extracellular protein or structure. Traditional static imaging of fixed bone cells and tissues takes a snapshot view of events at a specific time point, but can often miss the dynamic aspects of the events being investigated. This chapter provides an overview of the application of live cell imaging approaches for the study of bone cells and bone organ cultures. Rather than emphasizing technical aspects of the imaging equipment, we have focused on what we consider to be the important principles that are of most practical use for an investigator setting up these techniques in their own laboratory, together with detailed protocols that our laboratory has used for live imaging of bone cell and organ cultures.