Near-infrared dye loaded polymeric nanoparticles for cancer imaging and therapy and cellular response after laser-induced heating.
Near-infrared dye loaded polymeric nanoparticles for cancer imaging and therapy and cellular response after laser-induced heating.
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DOI：
10.3762/bjnano.5.35
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发表时间：
2014
影响因子：
3.1
通讯作者：
McGoron AJ
中科院分区：
文献类型：
--
作者：
Lei T;Fernandez-Fernandez A;Manchanda R;Huang YC;McGoron AJ
背景资料：在过去的十年中，研究人员一直致力于开发新的生物材料，用于癌症治疗，结合联合收割机成像和治疗剂。在我们的研究中，我们使用一种新的生物相容性和生物降解聚合物，称为聚（甘油苹果酸酯共十二烷二酸酯）（PGMD），用于纳米颗粒（NP）的合成和近红外（NIR）染料的负载。选择IR 820用于成像和热疗（HT）。HT目前用于与放疗和化疗联合治疗癌症的临床试验。HT的潜在问题之一是它可以上调缺氧诱导因子-1（HIF-1）的表达和增强血管内皮生长因子（VEGF）的分泌。结果如下：我们探索了快速，短期和低热剂量的激光-IR 820-PGMD NPs（激光/NPs）诱导加热后的细胞反应，并将其与细胞培养箱的缓慢，长期和高热剂量加热进行比较。两种不同加热模式后活性氧（ROS）、HIF-1和VEGF的表达水平。与孵育器HT相比，激光/NP HT后NP的细胞毒性导致更高的细胞杀伤。ROS水平在培养箱HT下高度升高，但在激光/NP HT下保持在基线水平。我们的结果表明，培养箱诱导HT后，细胞内ROS的表达增加可能导致HIF-1的表达增加。与激光/NP HT相比，VEGF分泌也显著增强，可能是由于HIF-1的促进。体外细胞成像和体内健康小鼠成像表明，IR 820-PGMD NPs可用于光学成像。结论：IR 820-PGMD纳米粒被开发并用于成像和治疗目的。快速和短期的激光/NP HT，具有低的热剂量，不上调HIF-1和VEGF的表达，而缓慢和长期的温箱HT，具有高的热剂量，增强这两种转录因子的表达。
Background: In the past decade, researchers have focused on developing new biomaterials for cancer therapy that combine imaging and therapeutic agents. In our study, we use a new biocompatible and biodegradable polymer, termed poly(glycerol malate co-dodecanedioate) (PGMD), for the synthesis of nanoparticles (NPs) and loading of near-infrared (NIR) dyes. IR820 was chosen for the purpose of imaging and hyperthermia (HT). HT is currently used in clinical trials for cancer therapy in combination with radiotherapy and chemotherapy. One of the potential problems of HT is that it can up-regulate hypoxia-inducible factor-1 (HIF-1) expression and enhance vascular endothelial growth factor (VEGF) secretion. Results: We explored cellular response after rapid, short-term and low thermal dose laser-IR820-PGMD NPs (laser/NPs) induced-heating, and compared it to slow, long-term and high thermal dose heating by a cell incubator. The expression levels of the reactive oxygen species (ROS), HIF-1 and VEGF following the two different modes of heating. The cytotoxicity of NPs after laser/NP HT resulted in higher cell killing compared to incubator HT. The ROS level was highly elevated under incubator HT, but remained at the baseline level under the laser/NP HT. Our results show that elevated ROS expression inside the cells could result in the promotion of HIF-1 expression after incubator induced-HT. The VEGF secretion was also significantly enhanced compared to laser/NP HT, possibly due to the promotion of HIF-1. In vitro cell imaging and in vivo healthy mice imaging showed that IR820-PGMD NPs can be used for optical imaging. Conclusion: IR820-PGMD NPs were developed and used for both imaging and therapy purposes. Rapid and short-term laser/NP HT, with a low thermal dose, does not up-regulate HIF-1 and VEGF expression, whereas slow and long term incubator HT, with a high thermal dose, enhances the expression of both transcription factors.
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DOI：
10.1073/pnas.1006646107
发表时间：
2010-11-23
影响因子：
11.1
作者：
Moon, Eui Jung;Sonveaux, Pierre;Dewhirst, Mark W.
通讯作者：
Dewhirst, Mark W.
影响因子：
4.9
作者：
Li, Shyh-Dar;Huang, Leaf
通讯作者：
Huang, Leaf
影响因子：
6
作者：
Dong, Z;Wang, JZ;Venkatachalam, MA
通讯作者：
Venkatachalam, MA
影响因子：
8.7
作者：
Madamanchi, NR;Li, SZ;Runge, MS
通讯作者：
Runge, MS
影响因子：
3.6
作者：
Brown, LM;Cowen, RL;Williams, KJ
通讯作者：
Williams, KJ