Fabrication of microstructures in photosensitive biodegradable polymers for tissue engineering applications
Fabrication of microstructures in photosensitive biodegradable polymers for tissue engineering applications
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DOI：
10.1016/j.biomaterials.2003.10.060
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发表时间：
2004-08-01
期刊：
影响因子：
14
通讯作者：
Fujii, T
中科院分区：
文献类型：
--
作者：
Leclerc, E;Furukawa, KS;Fujii, T
结合MEMS技术和组织工程的生物学要求，介绍了可生物降解光敏聚合物微结构腔体和微通道的制作工艺。基于软光刻的制造工艺非常快速和灵活。利用生物可降解聚合物可以获得各种单一和多步骤的微结构。可以制造出适合肝脏重建的50微米以下的微结构。由于pCLLA丙烯酸酯光敏聚合物具有植入式生物反应器的有趣特性，即它的柔软性，我们检测了各种哺乳动物细胞在其上生长和扩散的能力。以Hep G2细胞、人脐血管内皮细胞、3T3-L1小鼠成纤维细胞为研究对象，成功地在单个阶梯状微结构上进行静态培养。然后，利用这种光敏生物降解聚合物制备了具有简单流道的微结构，并进行了灌流实验。细胞培养和灌流实验都表明，本发明的光敏聚合物有可能作为可生物降解生物反应器的微流控载体用于植入应用。(C)2003爱思唯尔有限公司。保留所有权利。
Combining the MEMS technology and biology requirements for tissue engineering, the fabrication processes of microstructured chambers and microchannels made in biodegradable photosensitive polymers are presented. The fabrication processes, based on softlithography are very fast and flexible. Various single and multistepwise microstructures could be achieved using the biodegradable polymers. Microstructures down to 50 mum, which are suitable for liver reconstructs, could be fabricated. As the pCLLA acrylate photosensitive polymer has interesting property for implantable bioreactors, that is, its softness, we examined the ability of various mammalian cells to grow and spread on it. With Hep G2 cells, human umbilical blood vessel endothelial cells (HUVEC), 3T3-L1 mouse fibroblasts, static cultures could be successfully performed on single stepwise microstructures. Then, by using this photosensitive biodegradable polymer, a microstructure with simple fluidic channels is fabricated and a perfusion experiment could be carried out. Both cell cultures and perfusion experiments suggested the possibility to use the present photosensitive polymer as microfluidic supports for biodegradable bioreactors for implantation applications. (C) 2003 Elsevier Ltd. All rights reserved.