Genomic insights into the pathogenicity and environmental adaptability of Enterococcus hirae R17 isolated from pork offered for retail sale.
Genomic insights into the pathogenicity and environmental adaptability of Enterococcus hirae R17 isolated from pork offered for retail sale.
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从零售猪肉中分离出的海拉肠球菌 R17 的致病性和环境适应性的基因组学见解
DOI：
10.1002/mbo3.514
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发表时间：
2017-12
期刊：
影响因子：
3.4
通讯作者：
Li F
中科院分区：
文献类型：
--
作者：
Peng Z;Li M;Wang W;Liu H;Fanning S;Hu Y;Zhang J;Li F
关于鹑鸡肠球菌的遗传信息有限，这一特点影响了我们对这些具有临床挑战性的细菌的了解。在本研究中，对从中国北京一个零售市场购买的猪肉中分离出的一株达托霉素耐药菌株——鹑鸡肠球菌R17，以及其他三个肠球菌基因组（屎肠球菌DO、粪肠球菌V583和鹑鸡肠球菌ATCC™9790）进行了比较分析。鉴定出约1412个基因代表核心基因组，另外还有139个基因是鹑鸡肠球菌R17所特有的。这些R17菌株特异性编码序列的功能与碳水化合物转运和代谢以及转录的COGs类别相关，这一发现表明与其他三种细菌相比，鹑鸡肠球菌R17的碳水化合物利用能力可能更强。对基因组岛和毒力基因的分析凸显了水平基因转移在该分离株致病性变异中所起的作用。耐药基因预测和抗生素敏感性试验表明，鹑鸡肠球菌R17对几种抗菌化合物具有耐药性，包括杆菌肽、环丙沙星、达托霉素、红霉素和四环素，从而限制了化疗治疗选择。此外，对杀菌剂和金属的耐受性可能赋予一种表型，有助于该分离株在食品防腐剂、消毒剂和抗菌涂层环境下生存和适应。在鹑鸡肠球菌R17基因组中鉴定出的由插入序列元件、转座酶和串联重复序列介导的基因组可塑性，可能支持其对新环境生态位的适应，比如在住院患者体内发现的那些环境。一个预测的可传播质粒pRZ1被发现携带几种抗菌决定因子以及一些预测的致病基因。这些数据支持了先前确定的表型，证实食源的鹑鸡肠球菌R17是一种具有明显基因组可塑性和环境适应性的多重耐药致病菌。
Genetic information about Enterococcus hirae is limited, a feature that has compromised our understanding of these clinically challenging bacteria. In this study, comparative analysis was performed of E. hirae R17, a daptomycin‐resistant strain isolated from pork purchased from a retail market in Beijing, China, and three other enterococcal genomes (Enterococcus faecium DO, Enterococcus faecalis V583, and E. hirae ATCC ™9790). Some 1,412 genes were identified that represented the core genome together with an additional 139 genes that were specific to E. hirae R17. The functions of these R17 strain‐specific coding sequences relate to the COGs categories of carbohydrate transport and metabolism and transcription, a finding that suggests the carbohydrate utilization capacity of E. hirae R17 may be more extensive when compared with the other three bacterial species (spp.). Analysis of genomic islands and virulence genes highlighted the potential that horizontal gene transfer played as a contributor of variations in pathogenicity in this isolate. Drug‐resistance gene prediction and antibiotic susceptibility testing indicated E. hirae R17 was resistant to several antimicrobial compounds, including bacitracin, ciprofloxacin, daptomycin, erythromycin, and tetracycline, thereby limiting chemotherapeutic treatment options. Further, tolerance to biocides and metals may confer a phenotype that facilitates the survival and adaptation of this isolate against food preservatives, disinfectants, and antibacterial coatings. The genomic plasticity, mediated by IS elements, transposases, and tandem repeats, identified in the E. hirae R17 genome may support adaptation to new environmental niches, such as those that are found in hospitalized patients. A predicted transmissible plasmid, pRZ1, was found to carry several antimicrobial determinants, along with some predicted pathogenic genes. These data supported the previously determined phenotype confirming that the foodborne E. hirae R17 is a multidrug‐resistant pathogenic bacterium with evident genome plasticity and environmental adaptability.
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影响因子：
3.7
作者：
Cosentino S;Voldby Larsen M;Møller Aarestrup F;Lund O
通讯作者：
Lund O
影响因子：
4
作者：
Bourafa N;Loucif L;Boutefnouchet N;Rolain JM
通讯作者：
Rolain JM
DOI：
10.1016/j.ijfoodmicro.2007.11.067
发表时间：
2008-03-31
影响因子：
5.4
作者：
Abriouel, Hikmate;Ben Omar, Nabil;Galvez, Antonio
通讯作者：
Galvez, Antonio
影响因子：
8.4
作者：
Dortet, Laurent;Anguel, Nadia;Nordmann, Patrice
通讯作者：
Nordmann, Patrice
使用orthomcl将蛋白质分配给Orthomcl-DB组或将蛋白质组群集分为新的直系同源组。
影响因子：
--
作者：
Fischer, Steve;Brunk, Brian P;Chen, Feng;Gao, Xin;Harb, Omar S;Iodice, John B;Shanmugam, Dhanasekaran;Roos, David S;Stoeckert, Christian J Jr
通讯作者：
Stoeckert, Christian J Jr