Genome-scale comparison of expanded gene families in Plasmodium ovale wallikeri and Plasmodium ovale curtisi with Plasmodium malariae and with other Plasmodium species
Genome-scale comparison of expanded gene families in Plasmodium ovale wallikeri and Plasmodium ovale curtisi with Plasmodium malariae and with other Plasmodium species
复制标题
卵形疟原虫 wallikeri 和卵形疟原虫 curtisi 中扩展基因家族与三日疟原虫和其他疟原虫物种的基因组规模比较
DOI：
10.1016/j.ijpara.2016.05.009
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发表时间：
2016-10-01
影响因子：
4
通讯作者：
Pain, Arnab
中科院分区：
文献类型：
--
作者：
Ansari, Hifzur Rahman;Templeton, Thomas J.;Pain, Arnab
人类的疟疾是由六种疟原虫寄生虫引起的，其中两种卵形疟原虫（P. ovale curtisi 和 P. ovale wallikeri）以及三日疟原虫的核基因组序列尚未分析。在这里，我们对这三种寄生虫的核基因组序列进行了分析，并描述了其中的基因家族扩展。卵形疟原虫（Plasmodium ovale curtisi）和卵形疟原虫（P. ovale wallikeri）在遗传上不同，但在形态上难以区分，并且在非洲、亚洲和大洋洲的热带地区有同域分布。两种 P. ovale spp。显示 surfin 变体基因家族的扩展，以及疟原虫散布重复序列 (pir) 超家族的扩增，导致基因组大小增加约 30%。为了进行比较，我们还分析了三日疟原虫的核基因组草图，三日疟原虫是一种疟疾寄生虫，会引起轻微的疟疾症状，具有四分之一的生命周期、长期慢性感染和广泛的地理分布。三日疟原虫仅表现出中等水平的 pir 基因扩增，以及具有超过 550 个成员的高度分化的跨膜蛋白家族和配子 P25/27 基因家族的独特扩增。观察到的 P. ovale wallikeri 和 P. ovale curtisi 表面抗原的多样性，结合它们的系统发育分离，支持对这两种寄生虫给予物种地位的考虑。 (C) 2016 年作者。由爱思唯尔有限公司代表澳大利亚寄生虫学会出版。
Malaria in humans is caused by six species of Plasmodium parasites, of which the nuclear genome sequences for the two Plasmodium ovale spp., P. ovale curtisi and P. ovate wallikeri, and Plasmodium malariae have not yet been analyzed. Here we present an analysis of the nuclear genome sequences of these three parasites, and describe gene family expansions therein. Plasmodium ovale curtisi and P. ovale wallikeri are genetically distinct but morphologically indistinguishable and have sympatric ranges through the tropics of Africa, Asia and Oceania. Both P. ovale spp. show expansion of the surfin variant gene family, and an amplification of the Plasmodium interspersed repeat (pir) superfamily which results in an approximately 30% increase in genome size. For comparison, we have also analyzed the draft nuclear genome of P. malariae, a malaria parasite causing mild malaria symptoms with a quartan life cycle, long-term chronic infections, and wide geographic distribution. Plasmodium malariae shows only a moderate level of expansion of pir genes, and unique expansions of a highly diverged transmembrane protein family with over 550 members and the gamete P25/27 gene family. The observed diversity in the P. ovale wallikeri and P. ovale curtisi surface antigens, combined with their phylogenetic separation, supports consideration that the two parasites be given species status. (C) 2016 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology.