The photosynthesis characteristics of colored rice leaves and its relation with antioxidant capacity and anthocyanin content.
The photosynthesis characteristics of colored rice leaves and its relation with antioxidant capacity and anthocyanin content.
复制标题
DOI：
10.3864/j.issn.0578-1752.2022.03.004
复制
发表时间：
2022-01-01
影响因子：
--
通讯作者：
Tao, Long-xing
中科院分区：
文献类型：
--
作者：
Chen, Ting-ting;Fu, Wei-meng;Tao, Long-xing
目的：近年来，彩米因其独特的外观和特殊的营养价值，在观光农业、特殊营养米的选育和生产中得到了迅速发展。本研究的目的是阐明彩色水稻叶片对光的光合响应机制。方法：选取叶片叶绿素含量不同的4个水稻品种，分别为深紫（DP）、淡绿（PG）、深绿（DG）和淡紫（PP）。研究了叶片光合作用的变化特征，揭示了其响应机制。结果：叶绿素含量以DP最高，其次为DG和PP， PG最低。在这种情况下，PG品种的PSII实际荧光量子效率、光合速率、核酮糖-1,5-二磷酸羧化酶（Rubisco）活性和总干物质重均达到最高，显著高于其他品种。PG叶片过氧化氢和丙二醛含量显著低于其他水稻品种。DP与DG叶片叶绿素含量、类胡萝卜素含量无显著差异，但DP叶片PSII的实际荧光量子效率、净光合速率、Rubisco活性显著高于DG。相应的，DP叶片中抗氧化酶活性显著高于DG，而过氧化氢和丙二醛含量显著低于DG。这可能与叶片中的花青素含量有关，因为DP叶片中的花青素含量显著高于DG。叶面施用花青素后，叶片光合速率显著提高，过氧化氢含量显著降低。结论：推测花青素可能在缓解自然条件下过量光量子对叶片光合作用的抑制作用中发挥重要作用。研究结果可为水稻高光效育种和彩色水稻产量与品质协同发展的栽培技术开发提供理论依据。
Objective: In recent years, due to colored rice unique appearance and special nutritional value, it has been developed rapidly in the sightseeing agriculture, selection and production of special nutritional rice. The objective of this study was to elucidate the mechanism underlying the photosynthetic response of leaves in colored rice to light. Method: In this experiment, four rice varieties with different chlorophyll content in leaves were selected, including deep purple (DP), pale green (PG), dark green (DG) and pale purple (PP). The change characteristics of leaf photosynthesis were studied and the response mechanisms were revealed. Result: The results indicated that the highest chlorophyll content was showed in DP, followed by the DG and PP, while the lowest value was found in PG. Under this case, the PG variety attained the highest value of actual fluorescence quantum efficiency of PSII, photosynthetic rate, ribulose-1,5-bisphosphate carboxylase (Rubisco) activity and total dry matter weight, which were significantly higher than those in the other varieties. The content of hydrogen peroxide and malondialdehyde in PG leaves were significantly lower than those in other rice varieties. Besides, there was no significant difference in leaf chlorophyll content and carotenoid content between DP and DG, while the actual fluorescence quantum efficiency of PSII, net photosynthetic rate, Rubisco activity in leaves of DP were significantly higher than those of DG. Correspondingly, the activities of antioxidant enzymes in leaves of DP were significantly higher than those of DG, while the content of hydrogen peroxide and malondialdehyde in leaves of DP were significantly lower than those of DG. This might be related to the anthocyanin content in the leaves, because the anthocyanin content in the leaves of DP was significantly higher than that of DG. The leaf photosynthetic rate was significantly increased and hydrogen peroxide content was significantly decreased after foliar application of anthocyanin. Conclusion: It was inferred that anthocyanin might exert important role in alleviating the inhibition effect caused by excess optical quantum on the photosynthesis of leaves under natural conditions. The results could provide a theoretical basis for rice high light efficiency breeding, and cultivation technology exploitation for collaborative development in grain yield and quality of colored rice.