CELL FINE STRUCTURE AND BIOSYNTHESIS OF INTERCELLULAR MACROMOLECULES.
CELL FINE STRUCTURE AND BIOSYNTHESIS OF INTERCELLULAR MACROMOLECULES.
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细胞精细结构和细胞间大分子的生物合成。
DOI：
10.1016/s0006-3495(64)86936-8
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发表时间：
1964
影响因子：
3.4
通讯作者：
K. Porter
中科院分区：
文献类型：
--
作者：
K. Porter
在胶原蛋白生产中活跃的成纤维细胞显示出丰富的内质网（ER）和扩大的高尔基复合体，这两者都是参与蛋白质合成的细胞的特征。另一方面，相对静止的纤维细胞在这些相同的细胞质系统中是缺乏的。当成纤维细胞（或成软骨细胞）被提供氚化（H3）脯氨酸时，通过放射自显影，标签显示，首先与内质网池中的物质（胶原）结合，并及时转移到高尔基体，最终分泌到细胞外环境。硫酸软骨素与硫酸软骨素的结合似乎只涉及高尔基复合体的结构元素。有越来越多的证据表明，内源性成纤维细胞（或细胞）参与了单位胶原原纤维的形成和定向。这个问题在年轻的成年七鳃鳗突出的基底片的发育中被重新检查。
Fibroblastsactive in collagen production show a rich development of the endoplasmic reticulum (ER) and an enlarged Golgi complex, both characteristic of cells engaged in protein synthesis. The relatively quiescentfibrocyte,on the other hand, is deficient in these same cytoplasmic systems. When fibroblasts (or chondroblasts) are provided with tritiated (H3) proline, the label shows, by autoradiography, incorporation first into materials (collagen) in the cisternae of the ER, transfer thence in time to the Golgi, and eventual secretion into the extracellular environment. Sulfur25incorporation into chondroitin sulfate appears to involve only structural elements of the Golgi complex. There is increasing evidence of intimate fibroblast (or cell) involvement in the initiation and orientation of unit collagen fibrils. This question is reexamined in relation to the development of the prominent basement lamella of young adult lampreys.