Is sperminogen a modified proacrosin? Isolation, purification, and partial characterization of low-molecular-mass boar proacrosin.
Is sperminogen a modified proacrosin? Isolation, purification, and partial characterization of low-molecular-mass boar proacrosin.
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精蛋白原是修饰的顶体素原吗？
DOI：
10.1515/bchm3.1990.371.1.317
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
V. Jonáková
中科院分区：
文献类型：
--
作者：
D. Čechová;E. Töpfer‐Petersen;A. Zucker;V. Jonáková
用10%乙酸和10%甘油从猪精子中提取低分子量酶原和顶体酶原，并通过Sephadex G-75凝胶过滤和FPLC反相层析进行纯化。LMM酶原约占精子提取物中潜在胰蛋白酶样活性的5%。SDS-PAGE显示分子量为33 kDa。酶原与猪顶体酶的小鼠单克隆抗体和兔多克隆抗体均发生反应。N-末端34个氨基酸残基的序列测定表明其与已知的公猪顶体蛋白原的N-末端序列一致。
Low-molecular-mass zymogen was extracted from boar spermatozoa together with proacrosin using 10% acetic acid supplemented with 10% glycerol, and was purified by the sequential use of gel filtration on Sephadex G-75 and (FPLC) reversed-phase chromatography. LMM zymogen represented approximately 5% of the latent trypsin-like activity present in the sperm extract. SDS-PAGE indicated a molecular mass of 33 kDa. The zymogen reacted with both mouse monoclonal and rabbit polyclonal antibodies to boar acrosin. Determination of the N-terminal sequence of 34 amino-acid residues revealed its identity with the known N-terminal sequence of boar proacrosin.
与豚鼠精子顶体相关的三种主要蛋白酶的研究。
DOI：
10.1002/jez.1402440212
发表时间：
1987
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
Arboleda,CE;Gerton,GL
通讯作者：
Gerton,GL