Cellular interactions as a response to injury in the organ of Corti in culture
Cellular interactions as a response to injury in the organ of Corti in culture
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DOI：
10.1016/s0736-5748(96)00104-9
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发表时间：
1997-07-01
影响因子：
1.8
通讯作者：
Slapnick, SM
中科院分区：
文献类型：
--
作者：
Sobkowicz, HM;August, BK;Slapnick, SM
我们在激光束照射后的柯蒂氏器培养物中，对感觉细胞及其支持细胞之间的复杂关系进行了超微结构的发现和描述。使用波长为440nm的氮 - 染料脉冲激光对准内毛细胞的表皮板造成损伤。将激光损伤与用拉制的玻璃吸管对毛细胞区域造成的机械损伤进行了比较。无论损伤类型如何，但取决于其严重程度，存活的毛细胞可能：（1）失去其静纤毛，但仍存在于器官表面；（2）与网状板保持接触，但被支持细胞的突起过度生长；或者（3）与网状板隔离并被内化在支持细胞之间，在那里它们要么保持去分化状态，要么重新长出一个顶端突起，重新与器官表面接触。所有支持细胞，包括柱细胞和戴特斯细胞，都参与包裹它们各自的内毛细胞或外毛细胞。支持细胞不仅覆盖受伤的感觉细胞，还将其绒毛朝向受损的表皮板翻转，并在其周围释放细胞外基质。我们认为支持细胞在受伤毛细胞的恢复中起到保护和营养作用。（C）1997年国际标准连续出版物编号（ISDN可能有误，推测是指ISSN，国际标准连续出版物编号）
We discovered and described ultrastructurally the intricate relationships between the sensory cells and their supporting cells in cultures of the organ of Corti following laser beam irradiation. Injury was performed using a 440 nm nitrogen-dye pulse laser aimed at the cuticular plates of inner hair cells. Laser injury is compared with mechanical injury inflicted on the hair cell region by a pulled-glass pipette. Regardless of the type of injury, but depending on its severity, the surviving hair cells may: (1) lose their stereocilia but subsist at the surface of the organ; (2) retain contact with the reticular lamina but be overgrown by the processes of the supporting cells; or (3) become sequestered from the reticular lamina and internalized among the supporting cells, where they either remain dedifferentiated or regrow an apical process which regains contact with the surface of the organ. All supporting cells, including pillar and Deiters' cells, take part in wrapping their respective inner or outer hair cells. The supporting cells not only cover the injured sensory cells, but also invert their villi toward the maimed cuticular plates and release an extracellular matrix around them. We suggest that the supporting cells play a protective and trophic role in the recovery of injured hair cells. (C) 1997 ISDN.