In vivo inhibition of neuronal activity in the rat ventromedial prefrontal cortex by midbrain-raphe nuclei:: role of 5-HT1A receptors
In vivo inhibition of neuronal activity in the rat ventromedial prefrontal cortex by midbrain-raphe nuclei:: role of 5-HT1A receptors
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DOI：
10.1016/s0028-3908(03)00139-4
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发表时间：
2003-07-01
影响因子：
4.7
通讯作者：
Sharp, T
中科院分区：
文献类型：
--
作者：
Hajós, M;Gartside, SE;Sharp, T
内侧前额叶皮层腹侧部分在情绪和认知中起着重要作用。本研究通过测量腹侧mPFC神经元对电刺激中缝背核和正中核的电生理反应来研究5-HT在该区域的作用刺激DRN或MRN可在大多数腹侧mPFC神经元中引起一致的短潜伏期刺激后抑制（DRN：44/73神经元; MRN：24/31神经元）。有些神经元对DRN或MRN刺激有逆向棘波反应，表明它们是mPFC-中缝投射神经元。DRN-和MRN-诱发的抑制减弱全身给药的5-HT，，拮抗剂WAY 100635（0.1毫克/公斤静脉注射）。离子导入WAY 100635和全身给予5-HT 1A拮抗剂NAD-299（4 mg/kg静脉注射）也可以减弱DRN诱发的抑制作用而5-HT 2拮抗剂酮色林（4 mg/kg，i. v.）这些数据表明，DRN和MRN 5-HT神经元通过激活5-HT受体抑制腹侧mPFC中的神经元。其中一些mPFC神经元可能是5-HT 1A受体控制的DRN和MRN突触后反馈回路的一部分。(C)2003爱思唯尔科技有限公司版权所有。
The ventral part of the medial prefrontal cortex (mPFC) plays an important role in mood and cognition. This study examined the effect of the 5-HT in this region by measuring the electrophysiological response of ventral mPFC neurones to electrical stimulation of the dorsal and median raphe nuclei (DRN and MRN), which are the source of the 5-HT input.DRN or MRN stimulation evoked a consistent, short-latency, post-stimulus inhibition in the majority of ventral mPFC neurones tested (DRN: 44/73 neurones; MRN: 24/31 neurones). Some neurones responded to DRN or MRN stimulation with antidromic spikes indicating that they were mPFC-raphe projection neurones. Both DRN- and MRN-evoked inhibitions were attenuated by systemic administration of the 5-HT,, antagonist WAY 100635 (0.1 mg/kg i.v.). DRN-evoked inhibition was also attenuated by iontophoretic application of WAY 100635 and by systemic administration of the 5-HT1A antagonist, NAD-299 (4 mg/kg i.v.) but not the 5-HT2 antagonist ketanserin (4 mg/kg, i.v.).These data suggest that DRN and MRN 5-HT neurones inhibit neurones in the ventral mPFC via activation of 5-HT,, receptors. Some of these mPFC neurones may be part of a 5-HT1A receptor-controlled postsynaptic feedback loop to the DRN and MRN. (C) 2003 Elsevier Science Ltd. All rights reserved.