Reduced collagenase gene expression in fibroblasts from hypertrophic scar tissue
Reduced collagenase gene expression in fibroblasts from hypertrophic scar tissue
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DOI：
10.1046/j.1365-2133.1996.118853.x
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发表时间：
1996-05
影响因子：
10.3
通讯作者：
M. Arakawa;A. Hatmochi;Y. Mori;K. Mori;H. Ueki;T. Moriguchi
中科院分区：
文献类型：
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作者：
M. Arakawa;A. Hatmochi;Y. Mori;K. Mori;H. Ueki;T. Moriguchi
摘要增生性瘢痕病变的主要组织病理学特征是纤维化。以过度积累胶原蛋白为特征。本研究的目的是确定在增生性瘢痕成纤维细胞中是否不仅胶原表达增加，而且胶原酶表达减少。比较了增生性瘢痕不同部位成纤维细胞和正常真皮组织中α1（Ⅰ）、α1（Ⅲ）型胶原和胶原酶mRNA的表达。增生性瘢痕成纤维细胞。在瘢痕组织边缘和外部的成纤维细胞中观察到α1（I）和α1（III）胶原mRNA水平升高，而在该组织中心的成纤维细胞中观察到正常水平。相反。在增生性瘢痕成纤维细胞中发现胶原酶mRNA水平降低。中心（对照组的25%）的减少大于边缘（对照组的43%），大于外部（对照组的84%）。增生性瘢痕成纤维细胞的胶原酶mRNA水平的变化与胶原溶解活性的降低密切相关，如通过成纤维细胞培养上清液中荧光异硫氰酸酯标记的I型胶原的降解速率所确定的。这些结果表明，胶原酶在增生性瘢痕成纤维细胞中的表达减少可能是增生性瘢痕皮损中胶原过度积聚的原因之一。
Summary The major histopathological feature of hypertrophic scar lesions is fibrosis. characterized by excessive accumulation of collagen. The purpose of this study was to determine if there is not only increased expression of collagen but also decreased expression of collagenase in hypertrophic scar fibroblasts. We compared the expression of mRNA for of α1 (I) and α1 (III) collagen, and collagenase in cultured fibroblasis from different portions of hypertrophic scars and normal dermis. the hypertrophic scar fibroblasts. increased levels of α1 (I) and α1 (III) collagen mRNAs were observed in fibroblasts from the edge and outside of scar tissue, while normal levels were noted in fibroblasts from the centre of this tissue. In contrast. decreased levels of collagenase mRNA were found in the hypertrophic scar fibroblasts. The reductions were centre (25% of the control) greater than the edge (43% of the control) greater than the outside (84% of the control). The changes in the collagenase mRNA levels of the hypertrophic scar fibroblasts correlated well with decreased collagenolytic activity as determined by the degradation rate of fluorescent isothiocyanate‐labelled type I collagen in fibroblast culture supernatant. These results suggest that decreased expression of collagenase in hypertrophic scar fibroblasts may be one possible cause for the excessive accumulation of collagen in the skin lesions of hypertrophic scars.