The nef protein of the human immunodeficiency virus type 1 (HIV-1) inhibits a large-conductance potassium channel in human glial cells.
The nef protein of the human immunodeficiency virus type 1 (HIV-1) inhibits a large-conductance potassium channel in human glial cells.
复制标题
人类免疫缺陷病毒 1 型 (HIV-1) 的 nef 蛋白抑制人神经胶质细胞中的大电导钾通道。
DOI：
10.1016/s0304-3940(98)00495-9
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发表时间：
1998
影响因子：
2.5
通讯作者：
Jalonen,TO
中科院分区：
文献类型：
--
作者：
Kort,JJ;Jalonen,TO
人类免疫缺陷病毒1型（HIV-1）的Nef蛋白在HIV-1感染的星形胶质细胞中大量表达，并且与结合钾通道的α-蝎毒素具有氨基酸序列同源性。本文报道了重组Nef和α-蝎毒素对人星形胶质瘤U251细胞膜钾离子转运的影响，以86 Rb+为示踪剂，采用单通道膜片钳技术研究了细胞膜钾离子运动和钾通道活性。结果显示，当Nef被添加到U251细胞的由内而外的补丁的细胞膜的内小叶时，大电导钾通道失活。细胞外添加Nef或α-蝎毒素到完整的神经胶质细胞并不改变钾的转运。
The Nef protein of human immunodeficiency virus type 1 (HIV-1) is abundantly expressed in HIV-1-infected astrocytes and shares amino acid sequence homology to α-scorpion toxins which bind to potassium channels. Here we report effects of recombinant Nef and α-scorpion toxins on membrane potassium transport in human U251 astroglioma cells.86Rb+was utilized as a tracer for potassium movements and potassium channel activity was studied by single-channel patch-clamp techniques. Results showed inactivation of a large-conductance potassium channel when Nef was added to the inner leaflet of the cell membrane of inside-out patches of U251 cells. Extracellular addition of Nef or α-scorpion toxins to intact glial cells did not alter potassium transport.