Pretreatment with phytoestrogen-rich plant decreases breast tumor incidence and exhibits lower profile of mammary ERalpha and ERbeta.
Pretreatment with phytoestrogen-rich plant decreases breast tumor incidence and exhibits lower profile of mammary ERalpha and ERbeta.
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发表时间：
2007
期刊：
影响因子：
4.9
通讯作者：
W. Cherdshewasart;R. Panriansaen;P. Picha
中科院分区：
文献类型：
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作者：
W. Cherdshewasart;R. Panriansaen;P. Picha
目的植物雌激素在体外对人乳腺癌细胞具有抗增殖作用。我们测试了富含植物雌激素的葛根对大鼠体内乳腺癌诱导的影响。方法用0、10、100和1000 mg/kg BW/d剂量的奇异海盘车块茎粉连续4周灌胃给断乳雌性Spargue-Dawley大鼠。然后用单次剂量的7，12-DMBA（80 mg/kg BW）诱导乳腺肿瘤发展，随后每周检查乳腺肿瘤的大小和多样性，持续20周，最后进行尸检。研究乳腺组织的肿瘤毒力，并对ER α和ER β进行单克隆抗体染色。结果1000 mg/（kgBW·d）的奇异变形杆菌块茎粉预处理可降低大鼠肿瘤的毒力。乳腺肿瘤组织中ER α和ER β以及ER α/ER β的表达较低。结论奇异紫珠对7，12-DMBA诱导的大鼠乳腺肿瘤具有预防作用，其机制可能与其植物雌激素与ER α和/或ER α合成抑制因子的竞争性结合有关。
OBJECTIVE Phytoestrogens have been reported to exhibit antiproliferation to human breast cancer cells in vitro. We tested the phytoestrogen-rich, Pueraria mirifica against rat breast cancer induction in vivo. METHODS The weanling female Spargue-Dawley rats were pretreated with P. mirifica tuberous powder at a dosage of 0, 10, 100 and 1000 mg/kg BW/day for four consecutive weeks. Mammary tumor development was then induced with a single dose of 7,12-DMBA, 80 mg/kg BW, followed by a weekly examination for size and multiplicity of mammary tumors for 20 weeks and finally a necropsy. Mammary tissues were investigated for the virulence of tumor and also monoclonal antibody stained against ERalpha and ERbeta. RESULTS Pretreatment of 1000 mg/(kgBWday) of P. mirifica tuberous powder resulted in decreasing of the virulence of rat tumor development. The mammary tumor tissues exhibited lower profile of ERalpha and ERbeta as well as ERalpha/ERbeta. CONCLUSION P. mirifica exhibited prevention of 7,12-DMBA-induced rat mammary tumors, with a proposed mechanism of strong competitive binding of its phytoestrogens to ERalpha and/or synthesis suppressor of ERalpha.