Intermittent nutritional stimulus by short-term treatment of high-energy diet promotes ovarian performance together with increases in blood levels of glucose and insulin in cycling goats
Intermittent nutritional stimulus by short-term treatment of high-energy diet promotes ovarian performance together with increases in blood levels of glucose and insulin in cycling goats
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DOI：
10.1016/j.anireprosci.2010.09.005
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发表时间：
2010-12-01
影响因子：
2.2
通讯作者：
Kamomae, Hideo
中科院分区：
文献类型：
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作者：
Zabuli, Jahid;Tanaka, Tomomi;Kamomae, Hideo
本研究的目的是确定高能量饮食的短期间歇治疗是否对山羊卵巢功能和代谢状态有任何刺激作用。将骑自行车的柴山山羊分为试验组（TG组，n = 6）和对照组（CG组，n = 6）。通过超声检查检测排卵（第1次排卵，第0天）后，从第12天至第15天（4天）和第18天至第21天（4天）向TG饲喂高能量饮食（维持量的250%）。高能量饮食包括1000 g干草块和300 g浓缩饲料/头/天（约15 MJ的可消化能量/天）。在整个实验过程中，向CG提供维持饮食。在黄体期每隔一天进行经直肠超声检查，在卵泡期每天进行。在营养治疗后排卵后第-2天至第7天每天收集血液样品，用于分析血浆中的促卵泡激素（FSH）、孕酮、雌二醇、葡萄糖和胰岛素。葡萄糖和胰岛素浓度的两个波浪状上升出现在响应间歇性营养刺激。在每个治疗期的几乎所有天数，TG组的平均血糖和胰岛素浓度均显著（P < 0.05）高于CG组。对于卵巢性能，在第二次排卵前一天，TG中发育的卵泡总数（7.5 +/- 2.7）显著高于CG（4.5 +/- 2.6）。TG第二次排卵的排卵率（4.5 +/- 1.0）显著高于TG第一次排卵的排卵率（3.0 +/- 0.6），也高于CG第一次（2.5 +/- 0.5）和第二次（2.7 +/- 0.5）排卵的排卵率。在整个实验过程中，TG组和CG组的FSH、孕酮和雌二醇浓度无显著差异。本实验表明，从黄体期开始的短期间歇性营养刺激增加了排卵卵泡的总数和排卵率，并增加了山羊血浆葡萄糖和胰岛素浓度。(C)2010爱思唯尔有限公司版权所有。
The aim of this study was to determine if short-term intermittent treatments of high-energy diet have any stimulatory effects on ovarian function and metabolic status in goat. Cycling Shiba goats were divided into treatment (TG; n = 6) and control (CG; n = 6) groups. After the detection of ovulation (1st ovulation, Day 0) by ultrasonography, a high-energy diet (250% of maintenance) was fed to the TG from Day 12 to Day 15 (4 days) and from Day 18 to Day 21 (4 days). The high-energy diet comprised 1000 g hay-cubes and 300g of concentrated feed/head/day (approximately 15 MJ of digestible energy/day). The CG was offered maintenance diet throughout the experiment. Transrectal ultrasound examinations were conducted every other day during the luteal phase and were conducted daily during the follicular phase. Blood samples were collected daily from Day -2 to 7 days after ovulation (2nd ovulation) following the nutritional treatment for analysis of follicle stimulating hormone (FSH), progesterone, oestradiol, glucose and insulin in plasma. Two wave-like rises in the concentrations of glucose and insulin appeared in response to the intermittent nutritional stimulus. Mean plasma concentrations of glucose and insulin were significantly (P < 0.05) higher in the TG than in the CG on almost all days during each treatment period. For ovarian performance, the total number of follicles that developed on the day prior to the 2nd ovulation in the TG (7.5 +/- 2.7) was significantly higher than that of the CG (4.5 +/- 2.6). Ovulation rate of the 2nd ovulation (4.5 +/- 1.0) in the TG was significantly higher than that of the 1st ovulation (3.0 +/- 0.6) of the TG and than both 1st (2.5 +/- 0.5) and 2nd (2.7 +/- 0.5) ovulations of the CG. No significant difference was detected in the concentrations of FSH, progesterone and oestradiol throughout the experiment between TG and CG. The present experiment demonstrated that short-term intermittent nutritional stimulus from the luteal phase increased the total number of ovulatory follicles and the ovulation rate in association with increasing plasma concentrations of glucose and insulin in goats. (C) 2010 Elsevier B.V. All rights reserved.