Robiginitalea myxolifaciens sp. nov., a novel myxol-producing bacterium isolated from marine sediment, and emended description of the genus Robiginitalea.
Robiginitalea myxolifaciens sp. nov., a novel myxol-producing bacterium isolated from marine sediment, and emended description of the genus Robiginitalea.
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DOI：
10.1099/ijs.0.65598-0
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发表时间：
2008-07
影响因子：
2.8
通讯作者：
H. D. Manh;Y. Matsuo;Atsuko Katsuta;Satoru Matsuda;Y. Shizuri;H. Kasai
中科院分区：
文献类型：
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作者：
H. D. Manh;Y. Matsuo;Atsuko Katsuta;Satoru Matsuda;Y. Shizuri;H. Kasai
从日本冲绳收集的沉积物中分离出一种革兰氏阴性、深橙色、棒状/球形、严格需氧、不运动、氧化酶和过氧化氢酶阳性的海洋细菌菌株，命名为YM6-073(T)。菌株YM6-073(T)产生类胡萝卜素(3R, 2s)-myxol。基于16S rRNA基因序列的系统发育分析表明，菌株YM6-073(T)属于黄杆菌科一个独特的分支，其最近的亲缘关系为Robiginitalea biformata HTCC2501(T)（序列相似性为93.7%）。菌株YM6-073(T)可以通过许多表型特征与黄杆菌科的其他成员区分开来。DNA G+C含量为50.1 mol%。主要的细胞脂肪酸是iso-C（15:0）、iso-C(15:1) ω - 10c、iso-C(17:0) 3-OH和C(15:0)，唯一的呼吸醌是甲基萘醌-6。菌株YM6-073(T)与双形田鼠DSM 15991(T)的DNA-DNA杂交结果显示亲缘性小于10%。根据表型、基因型和系统发育特征，菌株YM6-073(T)为Robiginitalea myxolifaciens sp. nov.的新种，建议命名为Robiginitalea myxolifaciens sp. nov。型菌株为YM6-073(T) (=MBIC 06409(T)=KCTC 22216（T)=DSM 21019(T)）。
A marine, Gram-negative, deep-orange-coloured, rod/coccoid-shaped, strictly aerobic, non-motile, oxidase- and catalase-positive bacterial strain, designated YM6-073(T), was isolated from sediment collected in Okinawa, Japan. Strain YM6-073(T) produced the carotenoid (3R,2'S)-myxol. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YM6-073(T) belonged to a distinct lineage in the family Flavobacteriaceae, the closest relative being Robiginitalea biformata HTCC2501(T) (93.7 % sequence similarity). Strain YM6-073(T) could be distinguished from other members of the family Flavobacteriaceae by means of a number of phenotypic characteristics. The DNA G+C content was 50.1 mol%. The predominant cellular fatty acids were iso-C(15 : 0), iso-C(15 : 1)omega10c, iso-C(17 : 0) 3-OH and C(15 : 0,) and menaquinone-6 was the only respiratory quinone. DNA-DNA hybridization between strain YM6-073(T) and R. biformata DSM 15991(T) showed less than 10 % DNA-DNA relatedness. On the basis of phenotypic, genotypic and phylogenetic features, strain YM6-073(T) represents a novel species of the genus Robiginitalea, for which the name Robiginitalea myxolifaciens sp. nov. is proposed. The type strain is YM6-073(T) (=MBIC 06409(T)=KCTC 22216(T)=DSM 21019(T)).