HpaR, the Repressor of Aromatic Compound Metabolism, Positively Regulates the Expression of T6SS4 to Resist Oxidative Stress in Yersinia pseudotuberculosis.
HpaR, the Repressor of Aromatic Compound Metabolism, Positively Regulates the Expression of T6SS4 to Resist Oxidative Stress in Yersinia pseudotuberculosis.
复制标题
HpaR 是芳香族化合物代谢的阻遏蛋白，正向调节 T6SS4 的表达以抵抗假结核耶尔森氏菌的氧化应激。
DOI：
10.3389/fmicb.2020.00705
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发表时间：
2020
影响因子：
5.2
通讯作者：
Shen Xihui
中科院分区：
文献类型：
--
作者：
Wang Zhuo;Wang Tietao;Cui Rui;Zhang Zhenxing;Chen Keqi;Li Mengyun;Hua Yueyue;Gu Huawei;Xu Lei;Wang Yao;Yang Yantao;Shen Xihui
HpaR是一种马尔R家族转录调节因子，首次在大肠杆菌W中发现，可调节hpa-meta操纵子。关于它的功能知之甚少。在这里，我们报告说，在假结核耶尔森氏菌，HpaR负调控hpa-meta操纵子类似于在大肠杆菌。coli W.为了研究HpaR的其他功能，对野生型和ΔhpaR突变体进行了RNA测序，结果显示VI型分泌系统（T6 SS）受HpaR正调控。T6 SS 4是细菌抵抗环境胁迫，特别是氧化胁迫的重要因子。我们证明HpaR通过上调Y中T6 SS 4的表达来促进细菌抵抗氧化应激。假结核HpaR还参与生物膜形成、抗生素抗性、对真核细胞的粘附和小鼠中的毒力。这些结果极大地扩展了我们对HpaR功能的认识，并揭示了一种调节T6 SS 4的新途径。
HpaR, a MarR family transcriptional regulator, was first identified in Escherichia coli W for its regulation of the hpa-meta operon. Little else is known regarding its functionality. Here, we report that in Yersinia pseudotuberculosis, HpaR negatively regulates the hpa-meta operon similar to in E. coli W. To investigate additional functions of HpaR, RNA sequencing was performed for both the wild-type and the ΔhpaR mutant, which revealed that the type VI secretion system (T6SS) was positively regulated by HpaR. T6SS4 is important for bacteria resisting environmental stress, especially oxidative stress. We demonstrate that HpaR facilitates bacteria resist oxidative stress by upregulating the expression of T6SS4 in Y. pseudotuberculosis. HpaR is also involved in biofilm formation, antibiotic resistance, adhesion to eukaryotic cells, and virulence in mice. These results greatly expand our knowledge of the functionality of HpaR and reveal a new pathway that regulates T6SS4.