PHOTOSENSITIZATION OF CHINESE-HAMSTER CELLS BY WATER-SOLUBLE PHTHALOCYANINES
PHOTOSENSITIZATION OF CHINESE-HAMSTER CELLS BY WATER-SOLUBLE PHTHALOCYANINES
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DOI：
10.1111/j.1751-1097.1986.tb05636.x
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发表时间：
1986-06-01
影响因子：
3.3
通讯作者：
ROSENTHAL, I
中科院分区：
文献类型：
--
作者：
BENHUR, E;ROSENTHAL, I
酞菁是培养的哺乳动物细胞的有效光敏剂，并被认为用于光动力疗法。比较了酞菁磺酸氯铝（AlPCS）与未取代的水不溶性衍生物（AlPC）的光生物学性质。在生长培养基中加入染料后，光敏化的发展是这样的。AlPC比AlPCS快8倍。相反，当细胞在无染料的生长培养基中孵育一段时间的染料吸收后，光敏性的丧失对于AlPC也更快。染料吸收遵循类似于光敏性发展的动力学行为，但是染料的损失对于AlPC和AlPCS都太慢而不能解释光敏性的损失。当细胞在照射之前用磷酸盐缓冲盐水中的AlPCS而不是生长培养基孵育时，需要更短的时间和更少量的染料来实现相同水平的光敏化。AlPC和AlPCS的光敏性与染料浓度呈线性关系。正如已经发现的AlPC，光敏化的水溶性衍生物也没有增强D2O，这表明单线态氧不参与细胞毒性反应。水杨酸钠，这是发现，以提高效果的AlPC也有效与AlPCS。这种效应是非常特异的，因为Meta和帕拉异构体没有影响。与酞菁环络合的金属原子对于光生物活性是重要的。在所测试的化合物中，含有Al或Zn的化合物最具活性。
Phthalocyanines are efficient photosensitizers of cultured mammalian cells and are considered for use in photodynamic therapy. The photobiological properties of chloroaluminum phthalocyanine sulfonate (AlPCS) were compared to those of the unsubstituted, water-insoluble derivative (AlPC). The development of photosensitization after addition of the dye into growth medium is ca. 8 times more rapid for AlPC than for AlPCS. Conversely, the loss of photosensitization when cells are incubated in a dye-free growth medium following a period of dye uptake, is also faster for AlPC. The dye uptake followed a kinetic behavior similar to the development of photosensitivity, but the loss of dye was too slow for both AlPC and AlPCS to explain loss of photosensitivity. When cells are incubated prior to illumination with AlPCS in phosphate buffered saline instead of growth medium, shorter time and smaller amount of dye are required to achieve the same level of photosensitization. The dependence of photosensitivity on dye concentration is linear for both AlPC and AlPCS. As already found for AlPC, photosensitization by the water-soluble derivative is also not enhanced in D2O, suggesting that singlet oxygen is not involved in the cytotoxic response. Sodium salicylate, which was found to enhance the effect of AlPC was also effective with AlPCS. This effect is quite specific since the meta and para isomers had no effect. The metal atom complexed with the phthalocyanine ring is significant for the photobiological activity. Among the compounds tested, those containing Al or Zn are most active.