Connexin 43 expression promotes malignancy of HuH7 hepatocellular carcinoma cells via the inhibition of cell-cell communication
Connexin 43 expression promotes malignancy of HuH7 hepatocellular carcinoma cells via the inhibition of cell-cell communication
复制标题
DOI：
10.1016/j.canlet.2006.12.024
复制
发表时间：
2007-07-18
期刊：
影响因子：
9.7
通讯作者：
Morita, Ikuo
中科院分区：
文献类型：
--
作者：
Zhang, Dan;Kaneda, Makoto;Morita, Ikuo
在正常肝脏中，未检测到连接蛋白（Cx） 43，但在一些肝癌中表达上调。我们在此研究了Cx43在肝癌细胞癌变中的作用。使用shRNA沉默cx43的HuH7细胞生长速度较慢，分化程度较高，过表达cx43的细胞生长速度较快，分化程度较低。出乎意料的是，间隙连接细胞间通讯（GJIC）与Cx43水平呈负相关。此外，Cx32的表达水平和启动子活性受到Cx43表达的负调控。从这些数据来看，Cx43的表达可能通过降低由Cx32组成的GJIC而部分负责肝癌细胞的恶性。2007爱思唯尔爱尔兰有限公司版权所有。
In normal liver, Connexin (Cx) 43 is not detected, but up-regulated in some liver cancers. We herein investigated the role of Cx43 in hepatoma cell carcinogenesis. Cx43-silenced HuH7 cells using shRNA showed lower growth and higher differentiation, and Cx43-overexpressing cells exhibited rapid growth and low differentiation. Unexpectedly, gap junctional intercellular communication (GJIC) was inversely correlated with Cx43 levels. Furthermore, the expression level and promoter activity of Cx32 was negatively regulated by the expression of Cx43. From these data, Cx43 expression may be in part responsible for the malignancy of hepatoma cells through a decrease in GJIC composed of Cx32. (c) 2007 Elsevier Ireland Ltd. All rights reserved.