Human semen quality and sperm DNA damage in relation to urinary metabolites of pyrethroid insecticides
Human semen quality and sperm DNA damage in relation to urinary metabolites of pyrethroid insecticides
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DOI：
10.1093/humrep/den242
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发表时间：
2008-08-01
影响因子：
6.1
通讯作者：
Hauser, Russ
中科院分区：
文献类型：
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作者：
Meeker, John D.;Barr, Dana B.;Hauser, Russ
背景：对合成拟除虫菊酯杀虫剂的接触很普遍，预计在普通人群中会增加，因为在监管使用限制后，需要更换其他常见的杀虫剂。根据有限的研究，有动物和人类的证据表明，在接触拟除虫菊酯后，生殖或内分泌功能发生了变化。方法：采用中性彗星试验对2 0 7例男性不育症患者的尿拟除虫菊酯代谢产物[3-苯氧基苯甲酸(3PBA)、顺式和trans-3-(2，2-dichlorovinyl)-2，2-dimethylcyclopropane羧酸(CDCCA和TDCCA)]、精液质量、精子运动参数和精子DNA损伤进行检测。结果：在多变量分析中，与低于3PBA中位数的男性相比，最高的3PBA四分位数意味着精子密度降低了2020万精子/毫升(95%可信区间-37.1至+2.6)。在调整CDCCA和其他协变量后，TDCCA与精子活动率和精子运动参数呈显著负相关。与低于中位数的男性相比，TDCCA最高四分位数与精子活动率下降15.5%(95%可信区间-26.2至-4.8)相关。在多项Logistic分析中，与TDCCA相关的低于参考精子密度、活动率和形态的几率随剂量增加。在彗星检测措施中，3PBA和CDCCA与精子DNA损伤增加有关，以彗星尾部DNA百分比衡量。结论：我们发现精液质量下降和精子DNA损伤增加的证据与拟除虫菊酯类杀虫剂的尿代谢物有关。由于拟除虫菊酯使用量的增加和人类的普遍接触，这些发现可能令人担忧。
BACKGROUND: Exposure to synthetic pyrethroid insecticides is widespread, and is expected to increase among the general population due to the need to replace other common insecticides following regulatory use restrictions. On the basis of limited studies, there is animal and human evidence for altered reproductive or endocrine function following pyrethroid exposure. METHODS: The present study measured urinary pyrethroid metabolites [3-phenoxybenzoic acid (3PBA) and cis- and trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylic acid (CDCCA and TDCCA)], semen quality, sperm motion parameters and sperm DNA damage with the neutral comet assay in 207 men recruited from an infertility clinic. RESULTS: In multivariate analysis, the highest 3PBA quartile was associated with a suggestive 20.2 million sperm/ml reduction (95% confidence interval -37.1 to + 2.6) in sperm concentration compared with men below the 3PBA median. There were significant inverse associations between TDCCA and sperm motility and sperm motion parameters when adjusting for CDCCA and other covariates. The highest TDCCA quartile was associated with a 15.5% decline (95% confidence interval -26.2 to -4.8) in sperm motility compared with men below the median. In multiple logistic analyses, there were dose-dependent increased odds for below reference sperm concentration, motility and morphology in relation to TDCCA. Among the comet assay measures, 3PBA and CDCCA were associated with increased sperm DNA damage, measured as percent DNA in the comet tail. CONCLUSIONS:We found evidence for reduced semen quality and increased sperm DNA damage in relation to urinary metabolites of pyrethroid insecticides. These findings may be of concern due to increased pyrethroid use and prevalent human exposure.