Tβ4-overexpression based on the piggyBac transposon system in cashmere goats alters hair fiber characteristics
Tβ4-overexpression based on the piggyBac transposon system in cashmere goats alters hair fiber characteristics
复制标题
基于 PiggyBac 转座子系统的 Tbeta4 在绒山羊中过度表达会改变毛发纤维特性。
DOI：
10.1007/s11248-016-9988-7
复制
发表时间：
2017-02-01
影响因子：
3
通讯作者：
Chen, Yulin
中科院分区：
文献类型：
--
作者：
Shi, Bingbo;Ding, Qiang;Chen, Yulin
提高产绒量是绒山羊育种的重要目标之一。与传统的育种方法相比，转基因技术具有更高的效率，piggyBac（PB）转座子系统已被广泛应用于转基因动物的培育。在本研究中，供体成纤维细胞通过含有羊绒山羊胸腺素β 4（T β 4）编码序列的PB供体载体稳定转染，并由毛囊特异性启动子，角蛋白相关蛋白6.1（KAP6.1）启动子驱动。为了获得转基因细胞作为核供体，我们将供体载体共转染绒山羊胎儿成纤维细胞。利用体细胞核移植技术获得了5只转基因绒山羊。通过对T β 4基因拷贝数和整合位点的测定，成功地将其插入到山羊基因组中。皮肤组织的组织学检查显示，与野生型山羊相比，T β 4过表达的转基因山羊具有更高的次级毛囊与初级毛囊（S/P）比率。这表明T β 4过表达的山羊具有增加的次级毛囊（SHF）数量。我们的研究结果表明，在绒山羊中过表达T β 4可能是一种可行的策略，以提高产绒量。
Increasing cashmere yield is one of the vital aims of cashmere goats breeding. Compared to traditional breeding methods, transgenic technology is more efficient and the piggyBac (PB) transposon system has been widely applied to generate transgenic animals. For the present study, donor fibroblasts were stably transfected via a PB donor vector containing the coding sequence of cashmere goat thymosin beta-4 (T beta 4) and driven by a hair follicle-specific promoter, the keratin-associated protein 6.1 (KAP6.1) promoter. To obtain genetically modified cells as nuclear donors, we co-transfected donor vectors into fetal fibroblasts of cashmere goats. Five transgenic cashmere goats were generated following somatic cell nuclear transfer (SCNT). Via determination of the copy numbers and integration sites, the T beta 4 gene was successfully inserted into the goat genome. Histological examination of skin tissue revealed that T beta 4-overexpressing, transgenic goats had a higher secondary to primary hair follicle (S/P) ratio compared to wild type goats. This indicates that T beta 4-overexpressing goats possess increased numbers of secondary hair follicles (SHF). Our results indicate that T beta 4-overexpression in cashmere goats could be a feasible strategy to increase cashmere yield.