Imidacloprid-susceptible Nilaparvata lugens individuals exceeded resistant individuals in a mixture population with density pressure
Imidacloprid-susceptible Nilaparvata lugens individuals exceeded resistant individuals in a mixture population with density pressure
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在具有密度压力的混合群体中，吡虫啉敏感的褐飞虱个体超过了耐药个体
DOI：
10.1002/ps.4705
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发表时间：
2018
影响因子：
4.1
通讯作者：
Liu Zewen
中科院分区：
文献类型：
--
作者：
Yu Na;Tian Jiahua;Zhang Yixi;Li Zhong;Liu Zewen
背景与害虫杀虫剂抗性相关的适合度成本主要是在最佳实验室条件下研究的。然而，抗性昆虫在田间面临的压力不仅仅是杀虫剂，抗性种群对这些压力的反应对于害虫如褐飞虱的控制具有重要的实际意义。本研究的目的是探讨种群密度对抗感个体竞争力的影响。从田间抗性种群（HZ; RR 62.51）中创建了高抗性种群（HZ‐R）和相对敏感种群（HZ‐S），抗性比（RR）为227.10和RR为2.99。与HZ‐S相比，HZ‐R和HZ的高耐药水平主要归因于多个细胞色素P450（CYP）基因如CYP 6 ER 1、CYP 6AY 1、CYP 6CW 1和CYP 4CE 1的过表达，这得到增效增效作用的支持。与HZ和HZ-S相比，观察到HZ-R对噻虫啉和醚菊酯的抗性更高。最有趣的是，在高种群密度处理中，HZ-S个体比HZ-R个体更具竞争力。在密度压力下，鲁根的竞争力低于敏感的对应物。© 2017化学工业协会
BACKGROUNDFitness costs associated with insecticide resistance in pest insects have mainly been studied under optimal laboratory conditions. However, resistant insects face more stressors than just insecticides in the field, and how the resistant population reacts to these stressors is of practical importance for the control of pest insects such as the brown planthopperNilaparvata lugens. The aim of the present study was to explore the impact of population density on the competitiveness of resistant and susceptible individuals.RESULTSTwo isogenicN. lugenspopulations, a highly imidacloprid‐resistant population (HZ‐R) with a resistance ratio (RR) of 227.10 and a relatively susceptible population (HZ‐S) with an RR of 2.99, were created from a field‐resistant population (HZ; RR 62.51). The high resistance levels of HZ‐R and HZ were mainly attributable to the overexpression of multiple cytochrome P450 (CYP) genes such asCYP6ER1,CYP6AY1,CYP6CW1andCYP4CE1compared with HZ‐S, this being supported by piperonyl butoxide synergism. HZ‐R was observed to be more resistant to thiacloprid and etofenprox compared with HZ and HZ‐S. Most interestingly, in high population density treatments, HZ‐S individuals were much more competitive than HZ‐R individuals.CONCLUSIONImidacloprid‐resistant individuals ofN. lugensare less competitive than their susceptible counterparts under density pressure. © 2017 Society of Chemical Industry