Brain-Derived Neurotrophic Factor Prevents Low-Frequency Inputs from Inducing Long-Term Depression in the Developing Visual Cortex
Brain-Derived Neurotrophic Factor Prevents Low-Frequency Inputs from Inducing Long-Term Depression in the Developing Visual Cortex
复制标题
脑源性神经营养因子可防止低频输入在发育中的视觉皮层中引起长期抑郁
DOI：
10.1523/jneurosci.19-06-02122.1999
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
T. Tsumoto
中科院分区：
文献类型：
--
作者：
S. Kinoshita;H. Yasuda;N. Taniguchi;R. Katoh‐Semba;H. Hatanaka;T. Tsumoto
脑源性神经营养因子（BDNF）被报道可以增强突触传递，并在海马和新皮层的长时程增强中发挥作用。如果是这样的话，BDNF的缺乏或阻断可能会导致另一种形式的突触可塑性，即长期抑郁症（LTD）。为了测试这种可能性，并阐明其机制，如果是这样的话，EPSC诱发的测试刺激层IV记录从层II/III神经元在视觉皮层切片的年轻大鼠在全细胞电压钳模式。如果低频刺激（LFS）的每个脉冲与神经元的去极化配对至−30 mV，则以1 Hz持续10-15 min可诱导LTD，但如果其膜电位保持在−70 mV，则不会诱导LTD。这种LTD被外源性BDNF阻断，可能是通过突触前机制。抑制内源性BDNF活性的抗BDNF抗体或BDNF受体的抑制剂，否则无效的刺激（LFS无突触后去极化）有效的LTD诱导，这表明内源性BDNF可能会阻止低频输入诱导LTD在发展中的视觉皮层。
Brain-derived neurotrophic factor (BDNF) is reported to enhance synaptic transmission and to play a role in long-term potentiation in hippocampus and neocortex. If so, a shortage or blockade of BDNF might lead to another form of synaptic plasticity, long-term depression (LTD). To test this possibility and to elucidate mechanisms if it is the case, EPSCs evoked by test stimulation of layer IV were recorded from layer II/III neurons in visual cortical slices of young rats in the whole-cell voltage-clamp mode. LTD was induced by low-frequency stimulation (LFS) at 1 Hz for 10–15 min if each pulse of the LFS was paired with depolarization of neurons to −30 mV but was not induced if their membrane potentials were kept at −70 mV. Such an LTD was blocked by exogenously applied BDNF, probably through presynaptic mechanisms. Suppression of endogenous BDNF activity by the anti-BDNF antibody or an inhibitor for BDNF receptors made otherwise ineffective stimuli (LFS without postsynaptic depolarization) effective for LTD induction, suggesting that endogenous BDNF may prevent low-frequency inputs from inducing LTD in the developing visual cortex.
影响因子：
2.5
作者：
H. Scharfman
通讯作者：
H. Scharfman
DOI：
10.1073/pnas.92.17.8074
发表时间：
1995-08-15
影响因子：
11.1
作者：
LEVINE, ES;DREYFUS, CF;PLUMMER, MR
通讯作者：
PLUMMER, MR