Protective effects of Huangqin Decoction against ulcerative colitis and associated cancer in mice.
Protective effects of Huangqin Decoction against ulcerative colitis and associated cancer in mice.
复制标题
DOI：
10.18632/oncotarget.11426
复制
发表时间：
2016-09-20
期刊：
影响因子：
--
通讯作者：
Cao P
中科院分区：
文献类型：
--
作者：
Chen G;Yang Y;Hu C;Cheng X;Xu Y;Cai X;Wang M;Yang CS;Cao P
溃疡性结肠炎（UC）患者发展为结直肠癌（CRC）的风险很高。黄芩汤（HQD）是《商汉论》中记载的一种传统中药方剂，常用于治疗胃肠道症状。然而，缺乏支持临床实践的实验证据。本研究采用现代生物医学方法研究HQD对葡聚糖硫酸盐钠（DSS）诱导的小鼠急慢性UC和偶氮氧甲烷(AOM)/DSS诱导的CRC的保护/预防作用。采用4种不同的方法制备hqd: HQD-1和HQD-2在沸水中制备，HQD-3和HQD-4在加热乙醇（70%）中制备。对于HQD-1和HQD-3, 4种成分草药一起处理，而对于HQD-2和HQD4, 4种成分草药分别处理，然后组合处理。小鼠每日灌胃HQD 9.1 g/kg。HQD-1显著抑制dss诱导的急性UC，而HQD-3和HQD-4表现出轻微的改善作用；但HQD-2没有保护作用，死亡率较高。这种较高的死亡率可能是由于HQD-2比HQD-1中黄芩素和黄芩素的丰度更高。此外，HQD-1对dss诱导的慢性UC有保护作用，并显著抑制AOM/ dss诱导的小鼠CRC。HQD-1还能抑制慢性DSS和AOM/DSS治疗小鼠炎症细胞因子的产生，提高抗氧化能力。总的来说，HQD-1可能通过抑制炎症和防止氧化应激诱导的细胞损伤来抑制急性/慢性结肠炎的发展并预防结肠炎相关的结直肠癌。
Individuals with ulcerative colitis (UC) are at a high risk for developing colorectal cancer (CRC). Huangqin Decoction (HQD), a traditional Chinese medicinal formula chronicled in the Shang Han Lun, is commonly used to treat gastrointestinal symptoms. However, experimental evidence for supporting the clinical practice is lacking. This study used modern biomedical approaches to investigate the protective/preventive effects of HQD in dextran sulfate sodium (DSS)-induced acute/chronic UC and azoxymethane (AOM)/DSS-induced CRC in mice. HQDs were prepared in 4 different ways: HQD-1 and HQD-2 were prepared in boiling water, whereas HQD-3 and HQD-4 were prepared in heated ethanol (70%). For HQD-1 and HQD-3, the 4 constituent herbs were processed together, whereas for HQD-2 and HQD4, these herbs were processed individually and then combined. The mice were administered 9.1 g/kg HQD via oral gavage daily. HQD-1 significantly inhibited DSS-induced acute UC, whereas HQD-3 and HQD-4 exhibited mild ameliorative effects; but HQD-2 had no protective effect and resulted in a higher mortality rate. This higher mortality rate may be due to the greater abundance of baicalein and wogonin in HQD-2 than HQD-1. Furthermore, HQD-1 protected against DSS-induced chronic UC and significantly inhibited AOM/DSS-induced CRC in mice. HQD-1 also inhibited the production of inflammatory cytokines and increased antioxidant capacity both in chronic DSS and AOM/DSS treated mice. Overall, HQD-1 inhibits the development of acute/chronic colitis and prevents colitis-associated CRC, possibly by inhibiting inflammation and preventing oxidative stress induced cellular damage.