Genetic diversity assessed using PFGE, MLP and MLST in Candida spp. candidemia isolates obtained from a Brazilian hospital
Genetic diversity assessed using PFGE, MLP and MLST in Candida spp. candidemia isolates obtained from a Brazilian hospital
复制标题
DOI：
10.1007/s42770-021-00446-x
复制
发表时间：
2021-02-20
影响因子：
2.2
通讯作者：
da Silva Ferreira, Marcia Eliana
中科院分区：
文献类型：
--
作者：
Canela, Heliara Maria Spina;Cardoso, Barbara;da Silva Ferreira, Marcia Eliana
假丝酵母菌是免疫低下患者侵袭性真菌感染的主要病原体。念珠菌病的可归因性死亡率为15%至35%，并增加了住院时间和费用，因此使这种疾病成为一个公共卫生问题。本研究旨在利用脉冲场凝胶电泳法(PFGE)、微卫星长度多态性(MLP)和多位点序列分型技术(MLST)对65株念珠菌的遗传关系进行分析。血液分离株，包括35株白色念珠菌、15株光滑念珠菌和15株热带念珠菌，均来自巴西一家医院的患者。此外，对患者的临床数据进行了评估。所有技术都导致了较高的判别指数。白色念珠菌和热带念珠菌具有较高的遗传变异性，而光滑念珠菌的遗传变异性相对较低。此外，在一家医院的病房中发现了一群光滑念珠菌分离物。描述了新的MLST序列类型、二倍体序列类型和等位基因。未观察到分子分型结果与临床特征之间的关系。临床菌株的分子分型增加了我们对念珠菌病流行病学的了解，并促进了减少这种疾病发病率的策略的发展。此外，这项研究首次将这些技术结合在一起，在巴西对这三个物种进行了基因分型。
Candida spp. are the main causative agents of invasive fungal infections in immunocompromised patients. Candidemia has attributable mortality rates of 15 to 35% and increases hospitalisation time and costs, thus making this disease a public health concern. This study aimed to use pulsed-field gel electrophoresis (PFGE), microsatellite length polymorphism (MLP) and multilocus sequence typing (MLST) to analyse the genetic relationships among 65 Candida spp. bloodstream isolates, including 35 Candida albicans, 15 Candida glabrata and 15 Candida tropicalis isolates, all of which were obtained from patients in a Brazilian hospital. Moreover, patient clinical data were assessed. All techniques resulted in high discriminatory indexes. C. albicans and C. tropicalis isolates showed high genetic variability, while C. glabrata isolates had relatively low genetic variability. Moreover, a cluster of C. glabrata isolates was identified in a hospital unit. New MLST sequence types, diploid sequence types and alleles are described. Relationships were not observed between the molecular typing results and clinical characteristics. The molecular typing of clinical strains increases our understanding of candidemia epidemiology and promotes the development of strategies that can reduce the incidence of this disease. Moreover, this study is the first to combine these techniques to genotype these three species in Brazil.