Lack of luminal or basolateral uptake and transepithelial transport of mercury in isolated perfused proximal tubules exposed to mercury-metallothionein.
Lack of luminal or basolateral uptake and transepithelial transport of mercury in isolated perfused proximal tubules exposed to mercury-metallothionein.
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在暴露于汞-金属硫蛋白的隔离灌注近端小管中，汞的管腔或基底外侧摄取和跨上皮转运缺乏。
DOI：
10.1080/15287399509531946
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
D. Barfuss
中科院分区：
文献类型：
--
作者：
Rudolfs K. Zalups;M. Cherian;D. Barfuss
在兔肾近曲小管的分离灌注 S1、S2 和 S3 段中研究了与金属硫蛋白 (203Hg-MT) 结合的无机汞的腔到浴和浴到腔传输、细胞摄取和毒性。通过内腔灌注 Hg-MT 形式的 18.4 µM 无机汞的一些节段显示出非常轻微的毒性。毒性反应主要限于灌注移液管插入管腔的管状段末端的上皮细胞轻度肿胀。灌注 S2 节段近端部分的细胞似乎受到最严重的影响，因为一些泡有时会从上皮细胞上脱落。在一些 S2 和 S3 节段中也观察到轻度细胞肿胀，这些节段在浴中暴露于 Hg-MT 形式的 18.4 µM 无机汞。肿胀更为广泛，涉及沿灌注段的所有上皮细胞。在灌注 40-45 分钟期间，在近端小管的 3 个灌注段中的任何一个中都可以检测到非常少或没有可测量的 Hg 管腔到浴或浴到管腔的 Hg-MT 转运。 Hg-MT 复合物的行为似乎与体积标记 [3H]-L-葡萄糖的行为类似。通过肾小管上皮细胞中很少或没有可测量的无机汞的吸收和积累证实了汞作为 Hg-MT 的肾小管运输的缺乏。因此，我们的研究结果表明，Hg-MT 复合物在近端小管的隔离灌注 S1、S2 或 S3 段中并未被大量吸收。
The lumen-to-bath and bath-to-lumen transport, cellular uptake, and toxicity of inorganic mercury bound to metallothionein (203Hg-MT) were studied in isolated perfused S1, S2, and S3 segments of the renal proximal tubule of rabbits. Evidence of very mild toxicity was displayed in some of the segments perfused through the lumen with 18.4 microM inorganic mercury in the form of Hg-MT. The toxic response was restricted primarily to mild swelling of the epithelial cells localized at the end of the tubular segments where the perfusion pipette was inserted into the lumen. The cells in the proximal portions of perfused S2 segments appeared to be most severely affected in that a few blebs would on occasion come off the epithelial cells. Mild cellular swelling was also observed in some S2 and S3 segments that were exposed to 18.4 microM inorganic mercury in the form of Hg-MT in the bath. The swelling was more generalized, involving all the epithelial cells along the perfused segment. Very little, or no, measurable lumen-to-bath or bath-to-lumen transport of Hg as Hg-MT could be detected in any of the 3 perfused segments of the proximal tubule during 40-45 min of perfusion. The complex of Hg-MT appeared to behave in a manner similar to that of the volume marker [3H]-L-glucose. The lack of tubular transport of Hg as Hg-MT was confirmed by little or no measurable uptake and accumulation of inorganic mercury in the tubular epithelial cells. Thus, our findings indicate that the Hg-MT complex is not taken up avidly in isolated perfused S1, S2, or S3 segments of the proximal tubule.
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DOI：
10.1681/asn.v16910
发表时间：
1990
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
Barfuss,DW;Robinson,MK;Zalups,RK
通讯作者：
Zalups,RK
影响离体灌注近端小管中无机汞转运和毒性的因素。
DOI：
10.1681/asn.v24866
发表时间：
1991
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
Zalups,RK;Robinson,MK;Barfuss,DW
通讯作者：
Barfuss,DW
影响因子：
4.5
作者：
Zalups,RK;Cherian,MG;Barfuss,DW
通讯作者：
Barfuss,DW
DOI：
--
发表时间：
1988
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Clapp,WL;Park,CH;Madsen,KM;Tisher,CC
通讯作者：
Tisher,CC