Expandable Cardiovascular Progenitor Cells Reprogrammed from Fibroblasts.
Expandable Cardiovascular Progenitor Cells Reprogrammed from Fibroblasts.
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DOI：
10.1016/j.stem.2016.02.001
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发表时间：
2016-03-03
期刊：
影响因子：
23.9
通讯作者：
Ding S
中科院分区：
文献类型：
--
作者：
Zhang Y;Cao N;Huang Y;Spencer CI;Fu JD;Yu C;Liu K;Nie B;Xu T;Li K;Xu S;Bruneau BG;Srivastava D;Ding S
基于干细胞的心脏再生方法越来越成为治疗心力衰竭的可行策略。然而，在这种环境下生成丰富且有功能的自体细胞用于移植仍然是一个重大挑战。在这里，我们分离出在小鼠成纤维细胞心脏转分化过程中具有广泛增殖能力和有限心血管分化潜力的细胞群。这些诱导的可扩增心血管祖细胞 (ieCPC) 在化学限定的条件下广泛增殖超过 18 代，其中 105 个起始成纤维细胞稳健地产生 1016 个 ieCPC。 ieCPC 表达心脏特征基因，即使在长期扩增后，也能在体外轻易分化为功能性心肌细胞 (CM)、内皮细胞 (EC) 和平滑肌细胞 (SMC)。当移植到心肌梗塞后的小鼠心脏中时，ieCPC 会自发分化为 CM、EC 和 SMC，并在移植后长达 12 周内改善心脏功能。因此，ieCPC 是研究心血管规范并为心脏再生医学提供策略的强大系统。
Stem cell-based approaches to cardiac regeneration are increasingly viable strategies for treating heart failure. Generating abundant and functional autologous cells for transplantation in such a setting, however, remains a significant challenge. Here, we isolated a cell population with extensive proliferation capacity and restricted cardiovascular differentiation potentials during cardiac transdifferentiation of mouse fibroblasts. These induced expandable cardiovascular progenitor cells (ieCPCs) proliferated extensively for more than 18 passages in chemically defined conditions, with 105 starting fibroblasts robustly producing 1016 ieCPCs. ieCPCs expressed cardiac signature genes and readily differentiated into functional cardiomyocytes (CMs), endothelial cells (ECs), and smooth muscle cells (SMCs) in vitro, even after long-term expansion. When transplanted into mouse hearts following myocardial infarction, ieCPCs spontaneously differentiated into CMs, ECs, and SMCs and improved cardiac function for up to 12 weeks after transplantation. Thus, ieCPCs are a powerful system to study cardiovascular specification and provide strategies for regenerative medicine in the heart.
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影响因子：
2.8
作者：
Hirata, Hirokazu;Kawamata, Shin;Sawa, Yoshiki
通讯作者：
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影响因子：
23.9
作者：
Burridge, Paul W.;Keller, Gordon;Gold, Joseph D.;Wu, Joseph C.
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DOI：
10.1073/pnas.0605768103
发表时间：
2006-12-26
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
64.8
作者：
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通讯作者：
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影响因子：
64.8
作者：
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通讯作者：
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