Impaired Expression of Chloroplast HSP90C Chaperone Activates Plant Defense Responses with a Possible Link to a Disease-Symptom-Like Phenotype
Impaired Expression of Chloroplast HSP90C Chaperone Activates Plant Defense Responses with a Possible Link to a Disease-Symptom-Like Phenotype
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DOI：
10.3390/ijms21124202
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发表时间：
2020-06-01
影响因子：
5.6
通讯作者：
Kobayashi, Kappei
中科院分区：
文献类型：
--
作者：
Islam, Shaikhul;Bhor, Sachin Ashok;Kobayashi, Kappei
转基因烟草植物i-hpHSP 90 C的RNA-seq分析，其中叶绿体HSP 90 C基因可以以人工诱导的方式沉默，导致褪绿症的发展，分别揭示了2746和3490个基因的上调和下调。这些差异表达基因的基因本体分析表明，ROS反应基因的上调;先天免疫和细胞死亡途径的激活;以及参与光合作用、质体组织和细胞周期的基因的下调。通过台盼蓝染色和电解质渗漏试验证实细胞死亡，通过二氨基联苯胺染色证实H（2）O（2）产生。这些结果共同表明，HSP 90 C伴侣蛋白水平的降低导致植物发生褪绿病，主要是通过叶绿体和光合作用相关基因的全面下调，以及光依赖性活性氧的产生，然后激活免疫反应，包括细胞死亡。
RNA-seq analysis of a transgenic tobacco plant, i-hpHSP90C, in which chloroplast HSP90C genes can be silenced in an artificially inducible manner resulting in the development of chlorosis, revealed the up- and downregulation of 2746 and 3490 genes, respectively. Gene ontology analysis of these differentially expressed genes indicated the upregulation of ROS-responsive genes; the activation of the innate immunity and cell death pathways; and the downregulation of genes involved in photosynthesis, plastid organization, and cell cycle. Cell death was confirmed by trypan blue staining and electrolyte leakage assay, and the H(2)O(2)production was confirmed by diaminobenzidine staining. The results collectively suggest that the reduced levels of HSP90C chaperone lead the plant to develop chlorosis primarily through the global downregulation of chloroplast- and photosynthesis-related genes and additionally through the light-dependent production of ROS, followed by the activation of immune responses, including cell death.