SlGID1a Is a Putative Candidate Gene for qtph1.1, a Major-Effect Quantitative Trait Locus Controlling Tomato Plant Height
SlGID1a Is a Putative Candidate Gene for qtph1.1, a Major-Effect Quantitative Trait Locus Controlling Tomato Plant Height
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SlGID1a 是 qtph1.1 的假定候选基因，qtph1.1 是控制番茄株高的主要效应数量性状基因座
DOI：
10.3389/fgene.2020.00881
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发表时间：
2020
影响因子：
3.7
通讯作者：
Zejun Huang
中科院分区：
文献类型：
--
作者：
Xiaolin Liu;Wencai Yang;Jing Wang;Mengxia Yang;Kai Wei;Xiaoyan Liu;Zhengkun Qiu;Tong van Giang;Xiaoxuan Wang;Yanmei Guo;Junming Li;Lei Liu;Jinshuai Shu;Yongchen Du;Zejun Huang
株高是作物的重要农艺性状。番茄（Solanum lycopersicum）株高突变体的几个基因已被克隆。然而，很少有植株高度的数量性状基因已被确定在番茄。本研究在番茄中定位了7个控制株高的QTL。其中，qtph1.1（番茄株高1.1）、qtph3.1和qtph12.1为主效QTL，分别解释了15%、16%和12%的表型变异（R2）。qtph1.1进一步定位于1号染色体上的18.9 kb区间。基于注释的番茄基因组（版本SL2.50，注释ITAG2.40），编码GA受体SlGID 1a的Solyc 01 g 098390是推定的候选基因。qtph1.1位点的SlGID 1a基因包含单核苷酸多态性（SNP），其导致蛋白质序列中的氨基酸改变。含有qtph 1. 1位点的近等基因系（NIL-qtph 1.1）表现出比野生型（NIL-WT）更短的节间长度和细胞长度。外源GA 3处理不能挽救NIL-qtph 1.1的矮化表型。转录组分析和实时定量逆转录PCR（qPCR）结果表明，NIL-qtph 1.1和NIL-WT的茎中存在GA和生长素生物合成和信号转导相关基因的差异表达。这些结果为进一步了解番茄株高的分子调控机制奠定了基础。
Plant height is an important agronomic trait in crops. Several genes underlying tomato (Solanum lycopersicum) plant height mutants have been cloned. However, few quantitative trait genes for plant height have been identified in tomato. In this study, seven quantitative trait loci (QTLs) controlling plant height were identified in tomato. Of which, qtph1.1 (QTL for tomato plant height 1.1), qtph3.1 and qtph12.1 were major QTLs and explained 15, 16, and 12% of phenotypic variation (R2), respectively. The qtph1.1 was further mapped to an 18.9-kb interval on chromosome 1. Based on the annotated tomato genome (version SL2.50, annotation ITAG2.40), Solyc01g098390 encoding GA receptor SlGID1a was the putative candidate gene. The SlGID1a gene underlying the qtph1.1 locus contained a single nucleotide polymorphism (SNP) that resulted in an amino acid alteration in protein sequence. The near-isogenic line containing the qtph1.1 locus (NIL-qtph1.1) exhibited shorter internode length and cell length than the wild type (NIL-WT). The dwarf phenotype of NIL-qtph1.1 could not be rescued by exogenous GA3 treatment. Transcriptome analysis and real-time quantitative reverse transcription PCR (qPCR) showed that several genes related to biosynthesis and signaling of GA and auxin were differentially expressed in stems between NIL-qtph1.1 and NIL-WT. These findings might pave the road for understanding the molecular regulation mechanism of tomato plant height.