Higher-Level Production of Volatile Fatty Acids In Vitro by Chicken Gut Microbiotas than by Human Gut Microbiotas as Determined by Functional Analyses
Higher-Level Production of Volatile Fatty Acids In Vitro by Chicken Gut Microbiotas than by Human Gut Microbiotas as Determined by Functional Analyses
复制标题
根据功能分析确定，鸡肠道微生物群在体外产生的挥发性脂肪酸水平高于人类肠道微生物群
DOI：
10.1128/aem.00327-12
复制
发表时间：
2012-08-01
影响因子：
4.4
通讯作者：
Wang, Xin
中科院分区：
文献类型：
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作者：
Lei, Fang;Yin, Yeshi;Wang, Xin
本研究的目的是确定肠道微生物群的组成和功能之间的关系。在这里，我们比较了人类和鸡肠道微生物群的细菌组成和发酵代谢产物。定量PCR （qPCR）和16S rRNA基因V3区的454焦磷酸测序结果显示，人和鸡的微生物组成有显著差异，鸡盲肠微生物比人粪便微生物更具多样性。分析了批处理和恒化条件下各菌群对养分的需求。结果表明，鸡盲肠微生物群需要单糖和多肽来维持体外平衡生长，而人类粪便微生物群更喜欢多糖和蛋白质。鸡的微生物群也比人类的微生物群产生更高浓度的挥发性脂肪酸。我们的数据表明，由于盲肠独特的解剖结构，鸡盲肠中存在不同的可发酵底物，这可能为鸟类所需的高能量代谢物的生产提供了一个有利于微生物营养的环境。因此，肠道结构、营养、免疫和生活方式都有助于选择一个独特的细菌群落，产生对宿主有益的代谢物类型。
The aim of this study was to determine the relationship between the composition and function of gut microbiota. Here, we compared the bacterial compositions and fermentation metabolites of human and chicken gut microbiotas. Results generated by quantitative PCR (qPCR) and 454 pyrosequencing of the 16S rRNA gene V3 region showed the compositions of human and chicken microbiotas to be markedly different, with chicken cecal microbiotas displaying more diversity than human fecal microbiotas. The nutrient requirements of each microbiota growing under batch and chemostat conditions were analyzed. The results showed that chicken cecal microbiotas required simple sugars and peptides to maintain balanced growth in vitro but that human fecal microbiotas preferred polysaccharides and proteins. Chicken microbiotas also produced higher concentrations of volatile fatty acids than did human microbiotas. Our data suggest that the availability of different fermentable substrates in the chicken cecum, which exist due to the unique anatomical structure of the cecum, may provide an environment favorable to the nourishment of microbiotas suited to the production of the higher-energy metabolites required by the bird. Therefore, gut structure, nutrition, immunity, and life-style all contribute to the selection of an exclusive bacterial community that produces types of metabolites beneficial to the host.