Triplex DNA logic gate based upon switching on/off their structure by Ag+/cysteine
Triplex DNA logic gate based upon switching on/off their structure by Ag+/cysteine
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基于 Ag/半胱氨酸打开/关闭其结构的三重 DNA 逻辑门
DOI：
10.1039/c5an01371b
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发表时间：
2015
期刊：
影响因子：
4.2
通讯作者：
Ling Liansheng
中科院分区：
文献类型：
--
作者：
Xiao Zhiyou;Zhu Houya;Xin Aiping;Li Yubin;Ling Liansheng
Ag+和Cys（半胱氨酸）可以调节分子内三链DNA的形成，它们可以关闭或打开寡核苷酸5 ' -TAMRA-TTC TCT TCC TCT TCC TTC TGA CGA CAG TTG ACT CTT CCT TCT CCT TCT CTT- bhq -2-3 ‘ （Oligo 1）和3 ’ -GAA GGA AGA GGA AGA GAA-5 ' （Oligo 2）的荧光。基于这一原理，开发了Ag+和Cys传感器。Ag+传感器的线性范围为2.5 nM - 40 nM，检出限为1.8 nM，而Cys传感器的线性范围为10.0 nM - 120.0 nM，检出限为8.2 nM。此外，随着Ag+和Cys的交替加入，荧光是可逆的。以Ag+和Cys为输入，荧光强度为输出，构建了DNA逻辑门。DNA逻辑门很简单；并且具有响应速度快、可逆性好等特点。
The formation of intramolecular triplex DNA can be regulated by Ag+ and Cys (cysteine), which switch off/on the fluorescence of the oligonucleotides, 5′-TAMRA-TTC TCT TCC TCT TCC TTC TGA CGA CAG TTG ACT CTT CCT TCT CCT TCT CTT-BHQ-2-3′ (Oligo 1) and 3′-GAA GGA AGA GGA AGA GAA-5′ (Oligo 2). Based on this principle, sensors for Ag+ and Cys are developed. The sensor for Ag+ has a linear range of 2.5 nM–40 nM and a detection limit of 1.8 nM, whereas the sensor for Cys has a linear range of 10.0 nM–120.0 nM and a detection limit of 8.2 nM. Furthermore, the fluorescence is reversible with the alternate addition of Ag+ and Cys. We constructed a DNA logic gate using Ag+ and Cys as the input, and the fluorescence intensity as the output. The DNA logic gate is simple; moreover, it has a fast response and good reversibility.