OXIDATION OF SUCCINATE BY PARENCHYMAL-CELLS ISOLATED FROM RAT-LIVER

OXIDATION OF SUCCINATE BY PARENCHYMAL-CELLS ISOLATED FROM RAT-LIVER
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DOI:
10.1016/0014-5793(75)80858-1
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发表时间:
1975-01-01
期刊:
影响因子:
3.5
通讯作者:
HARRIS, RA
HARRIS, RA
中科院分区:
生物学3区
文献类型:
--
作者:
MAPES, JP;HARRIS, RA

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2.材料和方法采用Berry和Friend [6]的方法,经Cornell等的改进,从饥饿48小时的雄性Wistar大鼠(180-240 g)中分离肝细胞。[7]和Krebs等人[8]的一项建议。将分离的细胞洗涤三次并悬浮于Krebs-Henseleit盐水中。细胞干重
2. Materials and methodsIsolated hepatic cells were prepared from 48 hr starved male Wistar rats (180-240 g) by the method of Berry and Friend [6] with the modifications of Cornell et al.[7] and Krebs et al.[8]. The isolated cells were washed three times and suspended in Krebs-Henseleit saline. The dry weight of the cells