Concanavalin A induced suppressor cell activity and autorosette forming cells in chronic myeloid leukemia patients.
Concanavalin A induced suppressor cell activity and autorosette forming cells in chronic myeloid leukemia patients.
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DOI：
10.1038/bjc.1983.267
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发表时间：
1983-12
影响因子：
8.8
通讯作者：
Gangal, S G
中科院分区：
文献类型：
--
作者：
Somasundaram, R;Advani, S H;Gangal, S G
在本论文中，已试图调查抑制细胞活性的CML患者在第一次和随后的缓解，以研究抑制细胞活性和疾病的进展之间的关系。为此，我们研究了第1、2和3次缓解期CML患者外周血中Con A激活的抑制细胞抑制自体淋巴细胞PHA反应的能力，并与正常健康献血员进行了比较。还研究了Con A活化的细胞群与自体红细胞（ARFC）形成红细胞的能力。结果表明，当用Con A激活时，来自第1次（61.8 +/- 6.1%）、第2次（62.6 +/- 3.0%）和第3次（55.3 +/- 4.8%）缓解的CML患者的淋巴细胞显示出显著高于正常健康供体（36.5 +/- 1.9%）的抑制细胞活性。同样，第一缓解期淋巴细胞（23.3 +/- 4.7%）和第二缓解期淋巴细胞（25.3 +/- 4.2%）自发抑制细胞活性的产生也高于对照组（10.1 +/- 2.5%）。然而，在第3次缓解中，自发抑制细胞活性（14.5 +/- 3.2%）与对照组相当。因此，似乎更高的抑制细胞前体群体存在于缓解期CML患者中。然而，这与疾病的进展无关。CML患者在第一次缓解时也显示出与抑制细胞功能相关的ARFC百分比增加。在随后缓解的少数患者中检测的ARFC活性与对照组相当，尽管功能性抑制剂活性增加。
In the present paper attempts have been made to investigate suppressor cell activity in CML patients in first and subsequent remissions in order to study the relationship between suppressor cell activity and progression of the disease. For this purpose, the ability of Con A activated suppressor cells from peripheral blood of CML patients in 1st, 2nd and 3rd remission to suppress PHA response of autologous lymphocytes is investigated and compared with that of normal healthy donors. The ability of Con A activated cell population to form rosettes with autologous RBCs (ARFC) is also investigated. The results indicate that lymphocytes from CML patients in 1st (61.8 +/- 6.1%), 2nd (62.6 +/- 3.0%) and 3rd (55.3 +/- 4.8%) remissions show significantly high suppressor cell activity than normal healthy donors (36.5 +/- 1.9%) when activated with Con A. Similarly, generation of spontaneous suppressor cell activity was also higher in 1st (23.3 +/- 4.7%) and 2nd (25.3 +/- 4.2%) remission lymphocytes than controls (10.1 +/- 2.5%). In the 3rd remission however, the spontaneous suppressor cell activity (14.5 +/- 3.2%) was comparable to controls. Thus it appears that a higher suppressor cell precursor population is present in CML patients in remission. However, this could not be correlated with the progression of the disease. CML patients in 1st remission also revealed an increased percentage of ARFC which correlated with the suppressor cell function. The ARFC activity tested in a few patients in subsequent remissions was comparable with controls although functional suppressor activity was increased.