Inactivation of Escherichia coli by sonication at discrete ultrasonic frequencies
Inactivation of Escherichia coli by sonication at discrete ultrasonic frequencies
复制标题
DOI：
10.1016/s0043-1354(00)00121-4
复制
发表时间：
2000-10-01
期刊：
影响因子：
12.8
通讯作者：
Thompson, JE
中科院分区：
文献类型：
--
作者：
Hua, I;Thompson, JE
超声灭活大肠杆菌（Escherichia coli，E. coli）中的表达。探讨了功率强度、溶解气体和超声频率对超声杀菌效果的影响。E.在20 kHz的频率下，大肠杆菌的电催化行为表现出准一级动力学行为，并适度依赖于总功率和功率强度。当功率强度为4.6 ~ 74 W cm（-2）时，含氧溶液中的速率系数在0.031 ~ 0.046 min（-1）之间变化。在最高声强74 W cm时，最容易发生失活。研究了三种溶解气体：氧气、氩气和氩气/氧气混合物。溶解气体的性质不会强烈影响灭活系数的大小，其变化范围为k = 0.027 min（-1）至0.047 min（-1）。超声频率在205-1017 kHz范围内对E.大肠杆菌在充氧溶液中的灭活。速率系数在0.030 ~ 0.078min（-1）之间。大肠杆菌创新频率为205 kHz;速率系数（k = 0.078 min（-1））大于1071 kHz时的系数（k = 0.030 min（-1）），系数为2.6和358 kHz（k = 0.064 min（-1））也证明了比618 kHz（k = 0.041 min（-1））或1017 kHz更高的效率。(C)2000爱思唯尔科技有限公司版权所有。
The sonolytic inactivation of Escherichia coli (E. coli) is reported. The impact of power intensity, dissolved gas and ultrasonic frequency on the germicide effectiveness of sonification has been explored. The inactivation of E. coli exhibits pseudo-first order behavior, and depends moderately on total power and power intensity at a frequency of 20 kHz. The rate coefficients in an oxygenated solution vary from 0.031 to 0.046 min(-1) when the power intensity ranges from 4.6 to 74 W cm(-2) Inactivation occurs most readily at the highest sound intensity, 74 W cm Three dissolved gases were investigated: oxygen, argon, and an argon/oxygen mixture. The nature of the dissolved gas does not strongly influence the magnitude of the inactivation coefficients which varied from k = 0.027 min(-1) to 0.047 min(-1). Ultrasonic frequency, within the limits of 205-1017 kHz, displays a strong influence on the rate of E. coli inactivation in oxygenated solutions. The rate coefficients vary from 0.030 to 0.078 min(-1) The most effective ultrasonic frequency for E. coli innovation is 205 kHz; the rate coefficient (k = 0.078 min(-1)) is greater than the coefficient at 1071 kHz (k = 0.030 min(-1)) by a factor of 2.6 and 358 kHz (k = 0.064 min(-1)) also demonstrates a greater efficiency than either 618 kHz (k = 0.041 min(-1)) or 1017 kHz. (C) 2000 Elsevier Science Ltd. All rights reserved.