13C-NMR of the deoxyribose sugars in four DNA oligonucleotide duplexes: assignment and structural features.
13C-NMR of the deoxyribose sugars in four DNA oligonucleotide duplexes: assignment and structural features.
复制标题
四种 DNA 寡核苷酸双链体中脱氧核糖的 13C-NMR：分配和结构特征。
DOI：
10.1021/bi00175a011
复制
发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Borer,PN
中科院分区：
文献类型：
--
作者：
LaPlante,SR;Zanatta,N;Hakkinen,A;Wang,AH;Borer,PN
19939年11月24日摘要：获得了四种自互补DNA寡核苷酸[d（TAGCGCTA）] 2，[d（GGTATACC）] 2，[d（CG）3] 2和[d（TCGCG）] 2的天然丰度13 C-NMR谱，本文着重研究脱氧核糖共振。通过二维质子检测的异质结相关实验和ID光谱的比较，占31 P耦合，基地组成，和化学位移与温度曲线（5与T）的相似性相结合，进行了计算。双链体热解离后，观察到糖共振的大屏蔽和去屏蔽（在2.0和-1.9ppm之间）。8对T曲线的形状与这些双链体中连接在Cl'处的碱基的嘌呤/嘧啶性质强烈相关，所述双链体在交替的嘌呤-嘧啶序列内具有相当大部分的残基。这种相关性主要与呋喃糖假旋转态平衡分布的变化有关，这种变化可能部分来自于链间嘌呤-嘌呤空间冲突的缓解。
November 24, 19939 abstract: Natural-abundance 13C-NMR spectra have been obtained for four self-complementary DNA oligonucleotides:[d (TAGCGCTA)] 2,[d (GGTATACC)] 2,[d (CG) 3] 2, and [d (TCGCG)] 2; this paperfocuses on the deoxyribose resonances. Assignments were made by a combination of the two-dimensional proton-detected heteronuclear correlation experiment and comparison of ID spectra, accounting for 31P coupling, base composition, and similarities in chemical shift versus temperature profiles (5 vs T). Large shielding and deshielding of the sugar resonances(between 2.0 and-1.9 ppm) are observed upon thermal dissociation of the duplex. The shapes of the 8 vs T profiles correlate strongly with the purine/pyrimidine nature of the base attached at Cl'in these duplexes that have a substantial fraction of residues within alternating purine-pyrimidine sequences. The correlation is primarily associated with changes in the equilibrium distribution of furanose pseudorotational states that mayarise inpart from the relief of interstrand purine-purine steric clashes.