Studies on air-borne virus infections I. Experimental technique and preliminary observations on influenza and infectious ectromelia
Studies on air-borne virus infections I. Experimental technique and preliminary observations on influenza and infectious ectromelia
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DOI：
10.1017/s0022172400035828
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发表时间：
1943-01-01
影响因子：
--
通讯作者：
Laidlaw, PP
中科院分区：
文献类型：
--
作者：
Edward, DGF;Elford, WJ;Laidlaw, PP
I.描述了一种雾化病毒悬浮液的技术。大部分的展览馆。对甲型流感病毒P.R.8株进行了研究。病毒悬浮液用空气笔喷雾雾化，重量为35磅。平方。在……里面。压力。气雾剂被收集在一个大瓶子里，放在水或CaCl2上。通过小鼠滴定和通过连接到系统中的装有肉汤的已知大小的罐子的离心法进行病毒总数的估计。空气通过5%来苏和1%己基间苯二酚在50%酒精中的捕集器从系统中被置换出来。气雾剂(颗粒大小约2.3[亩])，通过水收集的迅速丧失感染力；通过CaCl2(约1.3[亩]直径。)保持传染性的时间要长得多。过滤排泄物。也说明了颗粒大小的重要性。暴露在感染环境中的小鼠感染流感的方式相当一致。吸入1/10稀释的500毫升混悬液可产生相当于5毫升全浓度物质鼻腔给药的感染。含有流感、牛痘和单纯疱疹病毒的气雾剂暴露于u-v。Hanovia灯或G.E.C.Sterilamp发出的光。气雾剂暴露在环形圆柱体或灯周围的螺旋容器中。吸入流感气雾剂的小鼠被杀死或肺部有明确的实变；暴露在辐射气雾剂中的小鼠只有一只存活。作者认为，辐射杀死了99%或更多的悬浮颗粒。从肉汤中采集痘苗病毒和疱疹病毒，用鸡绒毛膜-尿囊膜接种进行检测。牛痘病毒暴露于美国。亮一秒钟。将气雾剂消毒并照射0.5秒。达到90%的杀伤率。疱疹病毒气雾剂也得到了类似的结果。
I. A technique for atomizing virus suspensions is descr. Most of the exptl. work was carried out with influenza A virus, strain P. R. 8. The virus suspensions were atomized with an Aerograph Pencil Spray at 35 lbs. sq. in. pressure. The aerosols were collected in a large bottle over water or CaCl2. Estimations of virus conc, were made by mouse titration and by centrifugation of pots of known size, containing broth, which were connected into the system. Air was displaced from the system through a trap of 5% lysol and 1% hexyl-resorcinol in 50% alcohol. Aerosols (particle size approx. 2.3 [mu]) collected over water lost their infectivity rapidly; those collected over CaCl2 (approx. 1.3 [mu] diam.) remained infectious much longer. Filtration expts. also showed the importance of particle size. Mice exposed to infectious atmospheres contracted influenza in a rather uniform manner. Inhalation of 500 ml. of a 1/10 dilution of a suspension produced infection comparable to 5 ml. of full-strength material administered intranasally. Transmission of infectious ectromelia virus was also obtained.][long dash]II. Aerosols containing influenza, vaccinia and herpes simplex viruses were exposed to u.-v. light from a Hanovia lamp or from G. E. C. Sterilamp. The aerosols were exposed in an annular cylinder or in a spiral vessel around the lamp. Mice which inhaled influenza aerosol were killed or had definite consolidation of the lungs; only 1 mouse exposed to the irradiated aerosol survived. The authors considered that irradiation killed 99% or more of the suspended particles. Vaccinia and herpes viruses were collected in broth and tested by inoculation of chick chorio-allantoic membranes. Exposure of vaccinia virus to u.-v. light for one sec. sterilized the aerosol and irradiation for 0.5 sec. effected a 90% kill. Similar results were obtained with the herpes virus aerosol.