3′-processing of yeast tRNATrp precedes 5′-processing
3′-processing of yeast tRNATrp precedes 5′-processing
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DOI：
10.1261/rna.2145103
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发表时间：
2003-02-01
期刊：
影响因子：
4.5
通讯作者：
Tollervey, D
中科院分区：
文献类型：
--
作者：
Kufel, J;Tollervey, D
先前对真核前体tRNA加工的分析已经报道了RNase P的5 '-切割先于3'-成熟。在这里，我们报告说，与迄今为止分析的所有其他酵母tRNA相反，tRNA（Trp）在5 '-裂解之前经历3'-成熟。尽管其不寻常的加工途径，前-tRNA（TrP）类似于其他前-tRNA，显示依赖于必需的Lsm蛋白的正常加工和有效的协会与酵母La同系物，Lhp 1 p。tRNA T，p也不寻常，不需要Lhp 1 p进行3'加工和稳定性。然而，其他Lhp 1 p非依赖性tRNA，tRNA（2）（Lys）和tRNA（1）（Ile）遵循5 '-加工先于3-加工的正常途径。
Previous analyses of eukaryotic pre-tRNAs processing have reported that 5'-cleavage by RNase P precedes 3'-maturation. Here we report that in contrast to all other yeast tRNAs analyzed to date, tRNA(Trp) undergoes 3'-maturation prior to 5'-cleavage. Despite its unusual processing pathway, pre-tRNA(TrP) resembles other pre-tRNAs, showing dependence on the essential Lsm proteins for normal processing and efficient association with the yeast La homolog, Lhp1p. tRNA T,p is also unusual in not requiring Lhp1p for 3' processing and stability. However, other Lhp1p-independent tRNAs, tRNA(2)(Lys) and tRNA(1)(Ile) follow the normal pathway of 5'-processing prior to 3-processing.