COLLAGEN CHAIN MESSENGER-RNAS IN ISOLATED HEART-CELLS FROM YOUNG AND ADULT-RATS
COLLAGEN CHAIN MESSENGER-RNAS IN ISOLATED HEART-CELLS FROM YOUNG AND ADULT-RATS
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DOI：
10.1016/s0022-2828(88)80059-2
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发表时间：
1988-03-01
影响因子：
5
通讯作者：
BLUMENFELD, OO
中科院分区：
文献类型：
--
作者：
EGHBALI, M;CZAJA, MJ;BLUMENFELD, OO
胶原蛋白是心脏细胞外基质的主要成分，在那里它以层次结构的形式组织起来。为了确定不同类型胶原的细胞来源，对1~6月龄大鼠新鲜分离的心肌细胞和非心肌细胞心肌细胞的I型前胶原α2链、III型和IV型胶原的mRNAs进行了检测。心肌细胞形态完整，功能正常。非肌细胞组分以成纤维细胞样细胞为主，但也有内皮细胞和平滑肌细胞存在。以全脑室组织RNA为对照。Northern和斑点杂交分析用于确定mRNAs的存在或不存在。在从整个心室组织制备的RNA中，检测到α-、β-和γ-肌动蛋白亚型的mRNAs，而在心肌细胞中发现了α-肌动蛋白的mRNA，在非心肌细胞中发现了β-和γ-肌动蛋白的mRNAs，进一步证实了细胞群体的性质。心肌细胞总RNA中未检测到I型和III型前胶原基因，但有IV型胶原基因的表达。三种胶原蛋白的mRNAs均存在于非心肌细胞中。这些结果表明，在大鼠心脏中，非肌细胞细胞，可能是成纤维细胞，负责间质胶原的产生。这两种细胞群都可能参与基底膜IV型胶原的形成。
Collagen is the predominant component of the extracellular matrix of the heart, where it is organized in a hierarchy of structures. To establish the cellular origin of the various collagen types, type I-procollagen .alpha.2 chain and types III and IV collagen mRNAs were examined in preparations of myocytes and non-myocyte heart cells freshly isolated from rats 1 to 6 months old. The cardiomyocytes appeared mophologically intact and functionally competent. Fibroblast-like cells predominated in the non-myocyte cell fractions but endothelial and smooth muscle cells were also present. RNA from whole ventricular tissue served as a control. Northern and dot blot analyses were used to establish the presence or absence of mRNAs. In RNA prepared from whole ventricular tissue, the mRNAs for .alpha.-, .beta.-, and .gamma.-actin isotypes were detected whereas mRNA for .alpha.-actin was found in myocytes and those for .beta.- and .gamma.-actins were found in non-myocyte cells, confirming further the nature of the cell populations. Procollagen types I and III mRNAs were not detected in the total RNA of cardiomyocytes but mRNA for type IV collagen was present. The mRNAs for all three collagen types were present in the non-myocyte cells. These results suggest that in the rat heart the non-myocyte cells, probably fibroblasts, are reponsible for interstitial collagen production. Both cell populations may engage in the formation of basement membrane collagen type IV.