Changes in estrogenic regulation of estrogen receptor α mRNA and progesterone receptor mRNA in the female rat hypothalamus during aging:: an in situ hybridization study
Changes in estrogenic regulation of estrogen receptor α mRNA and progesterone receptor mRNA in the female rat hypothalamus during aging:: an in situ hybridization study
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DOI：
10.1016/s0168-0102(00)00150-4
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发表时间：
2000-09-01
影响因子：
2.9
通讯作者：
Mobbs, CV
中科院分区：
文献类型：
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作者：
Funabashi, T;Kleopoulos, SP;Mobbs, CV
我们研究了雌激素的两种分子反应：雌激素受体α（ER α）mRNA的减少和孕激素受体（PR）mRNA的增加，在下丘脑的3个月（年轻）和10个月大（中年）的周期，和15个月大（老）的非周期，Fischer 344雌性大鼠。切除大鼠卵巢，然后给予含有5% 17 β-雌二醇的硅橡胶胶囊或空植入物。并在植入后4天杀死。通过原位杂交的方法，我们发现。在幼年大鼠中，雌激素降低了下丘脑腹内侧核（VMH）和弓状核（ARC）的ER α mRNA，但不降低视前区（POA）的ER α mRNA。而雌激素对中老年大鼠VMH和ARC中ER α mRNA的影响无统计学意义。另一方面，在所有区域中，雌激素对PR mRNA的诱导在中年和老年大鼠中至少与年轻大鼠一样强。目前的研究表明，下丘脑中雌激素对PR mRNA的诱导并不随年龄的增长而受损，但VMH和ARC中的ER α mRNA随年龄的增长而显著受损：但POA中没有。(C)2000 Elsevier Science爱尔兰有限公司和日本神经科学学会。All rights reserved.
We examined two molecular responses to estrogen: reduction in estrogen receptor alpha (ER alpha) mRNA and increase in progesterone receptor (PR) mRNA, in the hypothalamus of 3- (young) and 10-month-old (middle-aged) cycling, and 15-month-old (old) acyclic, Fischer 344 female rats. The rats were ovariectomized and then given silastic capsules containing 5% 17 beta-estradiol: or empty implants. and killed 4 days after implantation. By means of in situ hybridization, we found that. in young rats, estrogen reduced ER alpha mRNA in both the ventromedial hypothalamus (VMH) and arcuate nucleus (ARC) but not in the preoptic area (POA). In contrast, the effect of estrogen on ER alpha mRNA in the VMH and ARC of middle-aged and old rats was not statistically significant. On the other hand in all regions the induction of PR mRNA by estrogen was at least as strong in middle-aged and old as in young rats. The present study revealed that the induction of PR mRNA by estrogen in the hypothalamus was not impaired with age but ER alpha mRNA in the VMH and ARC was significantly impaired with age: but not in the POA. (C) 2000 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.