BIOSYNTHESIS OF RIBOSYLTHYMINE IN TRANSFER-RNA OF STREPTOCOCCUS-FAECALIS - FOLATE-DEPENDENT METHYLATION NOT INVOLVING S-ADENOSYLMETHIONINE
BIOSYNTHESIS OF RIBOSYLTHYMINE IN TRANSFER-RNA OF STREPTOCOCCUS-FAECALIS - FOLATE-DEPENDENT METHYLATION NOT INVOLVING S-ADENOSYLMETHIONINE
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DOI：
10.1073/pnas.72.2.528
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发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
RABINOWITZ, JC
中科院分区：
文献类型：
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作者：
DELK, AS;RABINOWITZ, JC
在没有叶酸的情况下生长的粪链球菌的tRNA中不存在核糖基胸腺嘧啶，尽管当这种生物在叶酸存在的情况下生长时，这种甲基化残基确实存在于这种生物的tRNA中。我们发现，与粪肠球菌和其他生物核糖基化残基不同的是，叶酸充足的粪肠球菌tRNA的核糖基胸腺嘧啶的甲基部分不是来自S-腺苷蛋氨酸。初步证据表明，在这种甲基化中，叶酸衍生物是甲基供体。
Ribosylthymine is not present in tRNA of Streptococcus faecalis grown in the absence of folic acid, although this methylated residue does occur in the tRNA of this organism when it is grown in the presence of folate. We have found that, unlike other methylated residues in RNA of S. faecalis and other organisms, the methyl moiety of ribosylthymine of the tRNA of folate-sufficient S. faecalis is not derived from S-adenosylmethionine. Preliminary evidence suggests that a folate derivative serves as the methyl donor in this methylation.