Abscisic acid is involved in the response of grape (Vitis vinifera L.) cv. Malbec leaf tissues to ultraviolet-B radiation by enhancing ultraviolet-absorbing compounds, antioxidant enzymes and membrane sterols
Abscisic acid is involved in the response of grape (Vitis vinifera L.) cv. Malbec leaf tissues to ultraviolet-B radiation by enhancing ultraviolet-absorbing compounds, antioxidant enzymes and membrane sterols
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DOI：
10.1111/j.1365-3040.2009.02044.x
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发表时间：
2010-01-01
影响因子：
7.3
通讯作者：
Bottini, Ruben
中科院分区：
文献类型：
--
作者：
Berli, Federico J.;Moreno, Daniela;Bottini, Ruben
本文研究了脱落酸（阿坝）在葡萄叶片组织对不同紫外线（UV）-B处理的响应中的相互作用。一年生葡萄属欧亚种野生植株。暴露于光合有效辐射（PAR），其中通过使用聚酯过滤器消除太阳UV-B，或者其中PAR补充有UV-B照射。处理组合包括每周叶面喷洒阿坝或水对照。滤除UV-B后，吸收UV-B的黄酮醇、槲皮素和山奈酚的含量显著降低，而施加阿坝则增加了它们的含量。阿坝处理也增加了咖啡酸和阿魏酸的含量，但不受UV-B+处理的影响。类胡萝卜素水平和抗氧化酶，过氧化氢酶，抗坏血酸过氧化物酶和过氧化物酶的活性升高+阿坝处理，但只有当+UV-B。阿坝对细胞膜β-谷甾醇含量的增加与+UV-B无关。光保护化合物，抗氧化酶活性和甾醇的变化与减少膜伤害UV-B，离子泄漏评估。+UV-B处理下，以丙二醛含量表示的氧化损伤增加。我们的研究结果表明，葡萄叶片组织对UV-B辐射的防御系统被激活与阿坝作用的下游信号通路。
We investigated the interactions of abscisic acid (ABA) in the responses of grape leaf tissues to contrasting ultraviolet (UV)-B treatments. One-year-old field-grown plants of Vitis vinifera L. were exposed to photosynthetically active radiation (PAR) where solar UV-B was eliminated by using polyester filters, or where PAR was supplemented with UV-B irradiation. Treatments combinations included weekly foliar sprays of ABA or a water control. The levels of UV-B absorbing flavonols, quercetin and kaempferol were significantly decreased by filtering out UV-B, while applied ABA increased their content. Concentration of two hydroxycinnamic acids, caffeic and ferulic acids, were also increased by ABA, but not affected by plus UV-B (+UV-B) treatments. Levels of carotenoids and activities of the antioxidant enzymes, catalase, ascorbate peroxidase and peroxidase were elevated by +ABA treatments, but only if +UV-B was given. Cell membrane beta-sitosterol was enhanced by ABA independently of +UV-B. Changes in photoprotective compounds, antioxidant enzymatic activities and sterols were correlated with lessened membrane harm by UV-B, as assessed by ion leakage. Oxidative damage expressed as malondialdehyde content was increased under +UV-B treatments. Our results suggest that the defence system of grape leaf tissues against UV-B is activated by UV-B irradiation with ABA acting downstream in the signalling pathway.