ENHANCED INTRARENAL ANGIOTENSIN-II GENERATION IN RESPONSE TO OBSTRUCTION OF THE PIG URETER
ENHANCED INTRARENAL ANGIOTENSIN-II GENERATION IN RESPONSE TO OBSTRUCTION OF THE PIG URETER
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DOI：
10.1152/ajprenal.1992.263.3.f527
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发表时间：
1992-09-01
影响因子：
--
通讯作者：
DJURHUUS, JC
中科院分区：
文献类型：
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作者：
FROKIAER, J;KNUDSEN, L;DJURHUUS, JC
在猪模型上观察了急性单侧完全性输尿管梗阻(UUO)对肾脏血流动力学的影响。猪在全身麻醉下手术，肾动脉周围置入电磁流量探头，肾静脉和主动脉内置管采血。本文测定了麻醉动物阻断15h对血管紧张素Ⅱ(Ang II)免疫反应性生理变化的影响，并对Ang II的肾脏处理进行了详细的分析。盆腔压力在2小时内显著升高，最高达59.9+/-2.9cmH2O(n=10)。同侧肾血流量由319±35降至241±42ml/min，下降30%(P=0.001)，对侧肾血流量无明显变化。UUO后2小时内，平均动脉压有一过性显著升高。梗阻肾的肾血管阻力从37+/-3增加到55+/-9毫米汞，增加了44%。Ml-1。最小。G在阻塞的过程中。与此同时，三个样本部位的血浆Ang II免疫反应活性浓度均增加了8-15倍。与假手术组相比，这一增加非常显著。结果表明，猪肾脏对UUO的反应是通过同侧肾脏分泌Ang II来实现的。我们对Ang II肾脏分泌率的观察表明，肾静脉流出物中的部分Ang II免疫活性物质来自肾内生成。
The effect of acute unilateral complete ureteral obstruction (UUO) on renal hemodynamics has been examined in a pig model. Pigs were operated on under general anesthesia, electromagnetic flow probes were inserted around renal arteries, and catheters were implanted in renal veins and aorta for blood sampling. The influence of 15 h of obstruction on the physiological variation in immunoreactive angiotensin II (ANG II) was measured together with detailed analysis of the renal handling of ANG II in anesthetized animals. Pelvic pressure rose significantly within 2 h to a maximum of 59.9 +/- 2.9 cmH2O (n = 10). The ipsilateral renal blood flow (RBF) was reduced by 30% from 319 +/- 35 to 241 +/- 42 ml/min (P = 0.001), whereas contralateral RBF did not change. A temporary significant increase in mean aortic blood pressure was seen during the first 2 h after UUO. Renal vascular resistance increased by 44% in the obstructed kidney from 37 +/- 3 to 55 +/- 9 mmHg . ml-1 . min . g during the course of obstruction. Concomitantly, the plasma concentration of immunoreactive ANG II increased from all three sample sites by 8-15 times. This increase was highly significant compared with the sham-operated group. The results show that the pig kidney responds to UUO by a net secretion of ANG II from the ipsilateral kidney. Our observations on the ANG II renal secretion rate suggest that part of the immunoreactive ANG II in the renal venous effluent originated de novo from intrarenal generation.