Bis(2,4-dinitrophenyl) oxalate as a chemiluminescence reagent. Determination of fluorescent compounds by flow injection analysis
Bis(2,4-dinitrophenyl) oxalate as a chemiluminescence reagent. Determination of fluorescent compounds by flow injection analysis
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DOI：
10.1021/ac00257a028
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发表时间：
1983-05
影响因子：
7.4
通讯作者：
K. Honda;J. Sekino;K. Imai
中科院分区：
文献类型：
--
作者：
K. Honda;J. Sekino;K. Imai
利用二（2,4 -二氯苯基）草酸酯（DNPO）或二（2,4,6 -三氯苯基）草酸酯（TCPO）与过氧化氢的化学发光（CL）反应，检测缓冲溶液中的荧光化合物丹sylalanne （DNS-Ala）。研究了反应产物水、卤素盐、苯酚、2,4 -二硝基苯酚（DNP）和2,4,6 -三氯酚（TCP）等影响CL强度的因素。卤素极大地淬灭了这两种CL强度，反应介质中的含水量也起到了降低CL强度的作用。TCP对TCPO-CL反应中CL强度的影响较大，而DNP对DNPO-CL反应的影响较小。在DNS-Ala的流动注射分析中，DNPO-CL反应的敏感性优于TCPO-CL In。前一反应中DNS-Ala的检出限为5 fmol。
The chemiluminescence (CL) reaction of bis (2, 4-dlnltrophenyl) oxalate (DNPO) or bis (2, 4, 6-trlchlorophenyl) oxalate (TCPO) with hydrogen peroxide was used forthe detection of the fluorescent compound dansylalanlne (DNS-Ala) In buffer solution. Factors affecting the CL intensities, such as water, halogen salts, and phenols, 2, 4-dlnltrophenol (DNP) and 2, 4, 6-trlchlorophenol (TCP), which are reaction products, were studied. Halogen salts greatly quenched both CL In-tensities, and the water content In the reaction medium also acted to reduce them. While the effect of TCP on the CL Intensity In the TCPO-CL reaction was large, that of DNP in the DNPO-CL reaction was small. The DNPO-CL reaction was superior to that of TCPO-CL In sensitivity In the flow Injection analysis of DNS-Ala. The detection limit of DNS-Ala In the former reaction was 5 fmol.